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Tg(Gfap-flpo)62Thara - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Tg(Gfap-flpo)62Thara
transgene insertion 62, Toshiro Hara 		Driver: Gfap
Type: Transgenic (Recombinase)
Synonym: GFAP-FLPo
Molecular description: A transgene consist of an 15 kbp mouse glial fibrillary acidic protein (Gfap) sequence including its promoter region, regulatory elements, exons, introns and 3' UTR, as well as having a point mutation (ATG to TTG) in the Gfap start codon site to prevent GFAP expression from the transgene with an optimized FLP recombinase (flpo) followed by BGH polyA integrated into the Gfap first exon was used. The final transgene was used for pronuclear injection into B6D2F1/J x B6D2F1/J embryos. Founder animals were bred to C57BL/6J inbred mice for germline transmission. Founder line #62 was identified to have flpo expression predominantly in brain, and had the least amount of non-brain expression.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Tg(Gfap-flpo)62Thara activity also observed in:
head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory