|
|
|
Allele Information
|
|
Allele:
|
P2ry12em1(icre/ERT2)Tda
purinergic receptor P2Y, G-protein coupled 12;
endonuclease-mediated mutation 1, Thomas D Arnold
|
Driver: P2ry12
Type: Endonuclease-mediated
(Inducible, Recombinase)
|
|
|
Inducer:
|
tamoxifen
|
|
Synonym:
|
P2ry12-CreER, P2Y12-CreER
|
|
Molecular description:
|
The allele was created using CRISPR/cas9 endonuclease-mediated genome editing to target the stop codon of the P2ry12 locus on chromosome 3. The cas9-RNP complex and a 2A-iCreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the iCreER fusion gene with ~1.5 kbp homology arms) were injected into the pronucleus of C57BL/6N mouse embryos, followed by adoptive embryo transfer. Founder mice and progeny were screened by PCR, and proper genomic integration was verified by Southern blot and genomic sequencing. Founders were bred to C57BL/6J mice for germline transmission.
|
|
Find mice (IMSR):
|
Mouse Strains:
1
strain available
Cell Lines:
0 lines available
|
|
Additional Tissues:
|
P2ry12em1(icre/ERT2)Tda activity also observed in:
alimentary system,
head,
hemolymphoid system,
liver & biliary system,
nervous system,
respiratory system
|
|
|
Tissue Information
|
|
Cardiovascular System
|
Other recombinase alleles with activity in Cardiovascular System tissues:
A1cfTg(Myh6-cre/Esr1*)1Jmk,
Abcg2tm1.1(cre/Esr1*)Jvbe,
Aldh1l1em1(cre/ERT2)Nju,
Alpltm1(cre)Nagy,
Amhr2em1(icre)Fjd,
Amhr2tm3(cre)Bhr,
Angpt4tm1.1(cre)Ekl,
Aplnrem3(cre/ERT2)Smoc,
Aplntm1.1(cre/ERT2)Bzsh,
Atf3tm1.1(cre/ERT2)Msra,
Atoh8tm1(EGFP/cre)Gasa,
Calb2tm1(cre)Zjh,
Ccktm1.1(cre)Zjh,
Cd34tm1.1(cre/ERT2)Lizg,
Cdh5em1(cre/ERT2)Smoc,
Cdh5em1(nigri)Bzsh,
Cdkn2atm1.1(cre)Dvb,
Cdkn2atm1(cre/ERT2)Mkt,
Cdkn2atm2.1(cre/ERT2)Dvb,
Cdkn2atm3(cre)Cjs,
Cfap126tm1.1(icre)Heli,
Clutm2(EGFP/cre/ERT2)Wtsi,
Cntn2tm1.1(cre/EGFP)Nmun,
Col1a2em1(cre/ERT2)Bzsh,
Commd10Tg(Vav1-icre)A2Kio,
Crhtm1(cre)Zjh,
Crybb1em1(icre)Cln,
Cxcr5em1(cre/ERT2)Yhar,
Ddx4em1(icre)Jobo,
Dll4em1(cre/ERT2)Bz,
Dpttm1.1(cre/ERT2)Snjt,
E2f1Tg(Wnt1-cre)2Sor,
Egr1tm1.1(cre/ERT2)Jglt,
Eportm1.1(EGFP/icre)Uk,
Etv1tm1.1(cre/ERT2)Zjh,
Etv5tm1(cre/ERT2)Brst,
Fgf16tm1.1(cre)Sms,
Fgf18tm2.2(cre/ERT2)Dor,
Flt4tm2.1(cre/ERT2)Sgo,
Flt4tm2.1(cre/Esr1*)Ichi,
Fostm1.1(cre/ERT2)Luo,
Foxa2tm1(cre)Heli,
Foxa2tm2.1(cre/Esr1*)Moon,
Foxa2tm3.1(icre)Heli,
Foxd1tm1(GFP/cre)Amc,
Fstl1tm2.1(cre/ERT2)Ning,
Gad2tm2(cre)Zjh,
Gata4tm2(cre/ERT2)Ykaw,
Gfi1tm1(cre)Gan,
Gja5tm2(cre/ERT2,RFP)Lumi,
Gjd3tm1.1(cre/EGFP)Nmun,
Glp1rtm1.1(cre)Cors,
Gnao1tm2(EGFP/cre/ERT2)Wtsi,
Golm1tm2(EGFP/cre/ERT2)Wtsi,
Gpr4tm1(cre)Skas,
Gt(ROSA)26Sorem1(CAG-Ncre/vvd*,-vvd*/Ccre)Larc,
Gt(ROSA)26Sortm1.1(CAG-cre)Jphe,
Gt(ROSA)26Sortm14.1(Thy1-dre)Maoh,
Gt(ROSA)26Sortm1(cre/ERT2)Tyj,
Gt(ROSA)26Sortm2(cre/ERT2)Brn,
Gt(ROSA)26Sortm2(icre/ERT2)Jphe,
Gt(ROSA)26Sortm5(dre)Dwi,
Gt(ROSA)26Sortm8(CAG-dre/ERT2)Bzsh,
H2az2Tg(Wnt1-cre)11Rth,
Hand1tm1.1(EGFP/cre)Abfi,
Hand2em1(dre)Pjen,
Hcn4tm1(cre/ERT2)Anlu,
HhatTg(TFAP2A-cre)1Will,
Hic1tm2.1(cre/ERT2)Tmun,
Hlfem1(cre/ERT2)Smoc,
Hlfem2(cre/ERT2)Suda,
Hopxtm2.1(cre/ERT2)Joe,
Hopxtm4.1(cre)Joe,
Hprt1tm1(CAG-cre)Mnn,
Hprt1tm364a(CAG-icre/ERT2)Ems,
Hsd11b2tm1(cre)Anft,
Ifi208Tg(Cspg4-cre)1Akik,
Igs2tm1(Cdkn1a-cre/ERT2,-EGFP)Mxu,
Igs2tm2(Smarcd3-cre/ERT2)Bbr,
Isl1tm1(cre/Esr1*)Krc,
Isl1tm1(cre)Sev,
Isl1tm1(cre)Tmj,
Itga8tm1.1(cre/ERT2)Jomm,
Kdrtm1(cre)Sato,
Kittm1.1(cre)Jmol,
Kittm1(cre/ERT2)Bzsh,
Kittm1(cre/ERT2)Dsa,
Kittm2.1(cre/Esr1*)Jmol,
Krt17tm1(cre)Murr,
Krt19tm1(cre)Mmt,
Leprtm2(cre)Rck,
Lgals3em1(cre)Cya,
Lhx1tm1.1(icre)Rob,
Ltftm1(icre)Tdku,
Ly6atm1.1(cre/ERT2)Geno,
Ly6atm1.1(cre)Jmol,
Ly6atm1(cre/ERT2)Bzsh,
Ly6atm2.1(cre/Esr1*)Jmol,
Lyve1em3(cre/ERT2)Gpt,
Lyve1tm1.1(EGFP/cre)Cys,
Lyz2tm1(cre)Ifo,
Mcm2tm1(cre/ERT2)Scpr,
Mecomem1(cre/ERT2)Suda,
Mecomtm4.1(cre/ERT2)Aspe,
Meox1tm1(cre)Jpa,
Mfsd2aem1(cre/ERT2)Bzsh,
Mfsd2aem2(cre/ERT2)Bzsh,
Mgptm1.1(cre)Borr,
Mnx1tm4(cre)Tmj,
Mrc1em1(cre/ERT2)Mp,
Myf5tm3(cre)Sor,
Myh6em2(cre)Smoc,
Myh6tm1(cre)Bzsh,
Myl2tm1(cre)Krc,
Myocdtm1(cre)Dzw,
Myocdtm1(cre)Jomm,
Ndor1Tg(UBC-cre/ERT2)1Ejb,
Nfatc1em2(GFP/cre)Bzsh,
Nfatc1em3(dre)Bzsh,
Nfatc1em4(cre/ERT2)Bzsh,
Nfatc1tm1.1(cre)Bz,
Nfatc1tm1.1(cre/ERT2)Bzsh,
Nfatc1tm1(cre)Bz,
Nfatc1tm2(cre)Bzsh,
Nkx2-5tm1.1(cre/ERT2)Rard,
Nkx2-5tm1(cre)Rjs,
Nkx2-5tm2(cre)Rph,
Notch1tm3(cre)Rko,
Npr3em1(cre/ERT2)Bz,
Npr3em1(cre/ERT2)Bzsh,
Npr3tm2(cre/GFP)Bzsh,
Omptm4(cre)Mom,
Oprk1tm1.1(cre)Sros,
Osr2tm2(cre)Jian,
P2rx2tm1.1(cre)Ttc,
Pax3tm1(cre)Joe,
Pbx3tm1(EGFP/cre/ERT2)Wtsi,
Pdgfraem1(dre/ERT2)Bzsh,
Pdgfratm1.1(EGFP/cre/ERT2)Hyma,
Pdgfrbtm1.1(cre/ERT2)Csln,
Piezo1em2(cre/ERT2)Smoc,
Pitx2tm4(cre)Jfm,
Plxnd1tm2(EGFP/cre/ERT2)Wtsi,
Pmelem1(rtTA,tetO-icre)Osaw,
Prdm1tm3(icre)Rob,
Prdm1tm5.1(cre/ERT2)Rob,
Prlrtm1.1(cre)Uboe,
Pvalbtm1(cre)Arbr,
Rbpmsem1(cre/ERT2)Yzhe,
Runx1tm1(cre/Esr1*)Ims,
Runx1tm2.1(cre/Esr1*)Ims,
Sema3atm1.1(cre/ERT2)Bzsh,
Sema3dtm2.1(GFP/cre)Joe,
Setd4em1(cre)Wjy,
Sfrp5em1.1(ERT2/cre)Kkb,
Sfrp5tm1.1(cre)Kkb,
Shhtm1(EGFP/cre)Cjt,
Shroom3Gt(ROSA53)Sor,
Sox17tm1(icre)Heli,
Sox17tm2.1(icre)Heli,
Sox17tm4.1(icre/ERT2)Heli,
Sox9em1(cre/ERT2)Tchn,
Sptbtm2(EGFP/cre/ERT2)Wtsi,
Ssttm2.1(cre)Zjh,
Taglntm1(cre/ERT2)Feil,
Taglntm2(cre)Yec,
Tbx1tm4(cre/Esr1*)Bld,
Tbx1tm6(cre)Bld,
Tbx3em1(cre/ERT2)Toyo,
Tbx5tm1.1(cre/ERT2)Bbr,
Tcf7l1tm1.1(cre/ERT2)Hng,
Tekem1(dre)Bzsh,
Tg(Acp5-cre)4Rda,
Tg(ACTA1-cre)79Jme,
Tg(ACTA1-cre/Esr1*)2Kesr,
Tg(ACTA2-cre)1Miwa,
Tg(Acta2-cre)1Rkl,
Tg(Acta2-cre/ERT2)12Pcn,
Tg(Acta2-cre/ERT2)1Ikal,
Tg(Acta2-cre/ERT2)51Pcn,
Tg(ACTB-cre)1Tes,
Tg(ACTB-cre)#Bzsh,
Tg(ACTC1-cre)1Miwa,
Tg(ACTFLPe)9205Dym,
Tg(Acvrl1-cre)B1Spo,
Tg(Acvrl1-cre)L1Spo,
Tg(Aicda-cre,-CD2)1Hon,
Tg(Aicda-cre,-CD2)dME1Hon,
Tg(Akr1b7-cre)1Anm,
Tg(Alb1-cre)1Dlr,
Tg(Amigo2-icre/ERT2)1Ehs,
Tg(Aplnr-cre/ERT2)#Krh,
Tg(Bglap2-cre)2Kry,
Tg(Bmx-cre/ERT2)1Rha,
Tg(CAG-cre/ERT2/Hif1a)#Hhas,
Tg(CAG-cre/Esr1*)1Lbe,
Tg(CAG-cre/Esr1*)5Amc,
Tg(CAG-cre/Esr1*)86Lbgn,
Tg(Camk2a-cre)1Nobs,
Tg(Ccr2-cre/ERT2)12Kuan,
Tg(CD2-icre)4Kio,
Tg(Cdh5-cre)1Nmoc,
Tg(Cdh5-cre)1Spe,
Tg(Cdh5-cre)7Mlia,
Tg(Cdh5-cre/ERT2)1Rha,
Tg(Cdh5-cre/ERT2)CIVE23Mlia,
Tg(Cdh5-cre/ERT2)#Ykub,
Tg(CDX2-cre)101Erf,
Tg(Cdx2-cre)R337Npln,
Tg(Cga-cre)3Sac,
Tg(Chst4-cre)1Hkwa,
Tg(Ckmm-cre)5Khn,
Tg(Cldn11-cre/ERT2)151Tmak,
Tg(Clec4g-icre)1.1Sgoe,
Tg(CMV-cre)1And,
Tg(CMV-cre)1Cgn,
Tg(Col1a2-cre)23Angl,
Tg(Col1a2-cre/ERT,-ALPP)7Cpd,
Tg(Col2a1-cre)1Asz,
Tg(COL2A1-cre)1Wag,
Tg(Col6a1-cre)1Gkl,
Tg(Csf1r-icre)1Jwp,
Tg(Cspg4-cre)1Rkl,
Tg(Cspg4-cre/Esr1*)BAkik,
Tg(Csrp1-cre)1Sjc,
Tg(Ctgf-cre)2Aibs,
Tg(Cyp19a1-cre)5909Gle,
Tg(Cyp1a1-cre)1Dwi,
Tg(Cyp39a1-cre)1Aibs,
Tg(DBH-cre)9-9Koba,
Tg(Dbh-icre)1Gsc,
Tg(Ddx4-cre)1Dcas,
Tg(Dhh-cre)1Mejr,
Tg(Dll1-cre)33Dchp,
Tg(Dll4-cre)#Xia,
Tg(EIIa-cre)C5379Lmgd,
Tg(Eno2-cre)39Jme,
Tg(Epo-cre)44Mym,
Tg(Esm1-icre/ERT2)27Rha,
Tg(Etv2-cre)15508Djg,
Tg(Etv2-cre)#Blk,
Tg(Fabp4-cre)1Abel,
Tg(Fabp4-cre)1Rev,
Tg(Fabp4-cre/ERT2)1Ipc,
Tgfb3tm1(cre)Vk,
Tg(Fgf1-RFP,-cre/ERT2)13AChiu,
Tg(Fgfr3-icre/ERT2)4-2Wdr,
Tg(Flt1-cre/ERT2)1Tmak,
Tg(Frzb-cre)1Tylz,
Tg(Fzd9-cre)1Chzh,
Tg(Gata1-cre)1Sho,
Tg(Gata4*G2-cre)#Roja,
Tg(GATA5-cre)1Krc,
Tg(GATA6-cre)#Jbeb,
Tg(GFAP-cre)25Mes,
Tg(Gfap-cre)73.12Mvs,
Tg(Gfap-cre)77.6Mvs,
Tg(Gjb6-cre/ERT2)53-33Fwp,
Tg(Glp1r-icre)#Fmgb,
Tg(Gm5127-cre/ERT2)1Mhsi,
Tg(Grik4-cre)G32-4Stl,
Tg(GZMB-cre)1Jcb,
Tg(Hand1-EGFP/cre)#Abfi,
Tg(Hand2-cre)7-1Clou,
Tg(Hcn4-cre/ERT2)1Yzhao,
Tg(Hdnr-icre)5Phg,
Tg(Hsp70-1-cre)6Arge,
Tg(Hspa2-cre)1Eddy,
Tg(Ins2-cre)25Mgn,
Tg(Itga2b-cre)NE1/21Emam,
Tg(Kcne1-cre/ERT2)1Imos,
Tg(Kdr-cre)15962Brei,
Tg(Kdr-cre)15964Brei,
Tg(KRT14-cre)1Amc,
Tg(KRT14-cre/ERT)20Efu,
Tg(Leftb-cre)1Hmd,
Tg(LPAR4-cre/ERT2,-EGFP)#Hjcho,
Tg(Lyve1-cre/ERT2)49Rtem,
Tg(Mbp-cre)9Gvn,
Tg(Mbp-cre,CAG-EGFP)53Masa,
Tg(Mef2c-cre)2Blk,
Tg(Mef2c-cre)#Blk,
Tg(Mef2c-dre/ERT2)#Bbr,
Tg(MMTV-cre)4Mam,
Tg(Mpz-cre)94Imeg,
Tg(Mrgprb2-EGFP/cre)#Xzd,
Tg(Mx1-cre)1Cgn,
Tg(Mx1-cre)29-4Her,
Tg(Myh11-cre)5013Gko,
Tg(Myh11-cre,-EGFP)2Mik,
Tg(Myh11-cre/ERT2)F31Gko,
Tg(Myh11-dre/ERT2)#Cya,
Tg(Myh11-icre/ERT2)1Soff,
Tg(Myh6-cre)1Xya,
Tg(Myh6-cre)2182Mds,
Tg(Myh6-cre/ERT2/Hif1a)#Hhas,
Tg(Myh6-cre/PGR*)1Mds,
Tg(Myh6-cre/PGR*)6297Mds,
Tg(Myh7-cre)1Jmk,
Tg(Myh7-cre)1Zli,
Tg(myl7.L-cre)1118Tmhn,
Tg(myl7.L-cre)1141Tmhn,
Tg(MYOD1-cre)M23Glh,
Tg(Nes-cre)1Atp,
Tg(Nes-cre)1Kln,
Tg(Nkx2-5-cre)9Eno,
Tg(NKX2-5-cre)#Kyuhl,
Tg(NKX2-5*-cre)#Kyuhl,
Tg(Nkx2-5-tTA,tetO-EGFP/cre)1Smwu,
Tg(Nppa-cre)3Vmc,
Tg(Nppa-cre)4Vmc,
Tg(Nrp2-cre,-EGFP)#Qsch,
Tg(Ntrk3-cre/ERT2)#Phep,
Tg(Osr1-cre)4Mrt,
Tg(Pax3-cre)1Joe,
Tg(Pcdh21-cre)BYoko,
Tg(Pcp2-cre)1Amc,
Tg(Pcp2-cre)2Mpin,
Tg(Pdgfb-icre/ERT2,-EGFP)1Frut,
Tg(Pdgfrb-FLP1,Cspg4-cre/ERT2)#Bvz,
Tg(Pdpn-cre)HRkl,
Tg(Pf4-icre)Q3Rsko,
Tg(Pitx2-cre/ERT2)#Jfm,
Tg(Pitx2-cre/Esr1*)#Hmd,
Tg(Plp1-cre/ERT)3Pop,
Tg(Postn-cre)1Sjc,
Tg(Postn-icre/ERT2)#Wet,
Tg(Prdm1-cre)1Masu,
Tg(Prg4-cre/ERT2)#Ndy,
Tg(Prox1-cre/ERT2)1Tmak,
Tg(Prrx1-cre)1Cjt,
Tg(RARE/Hspa1b-cre/ERT2)#Asc,
Tg(Relb-cre)53Rie,
Tg(REN-cre)1Haca,
Tg(Rorc-cre)1Litt,
Tg(Rr141-cre)1Ksec,
Tg(Rr393-cre)#Sav,
Tg(Rr437-cre)#Hmd,
Tg(rtetR-tetO-cre)1Mhz,
Tg(Runx1/Hspa1a-cre/ERT2)#Itoy,
Tg(S100a4-cre)1Egn,
Tg(S100b-cre)20Ito,
Tg(Scx-cre)HShuk,
Tg(Sftpa1-cre)1Xya,
Tg(Sim1-cre)1Lowl,
Tg(Slco1c1-icre/ERT2)1Mash,
Tg(Smad7-cre)1Sjc,
Tg(Smarcd3-cre/ERT2)#Bbr,
Tg(Sox10-cre)1Wdr,
Tg(Sox10-cre)507Mcln,
Tg(Sox10-ERT2/cre/ERT2)17Sor,
Tg(Stab2-icre)1.2Cger,
Tg(Tagln-cre)1Her,
Tg(Tagln-cre)1Jjl,
Tg(Tagln-cre)1Jomm,
Tg(Tagln-cre)1Llw,
Tg(Tagln-cre/ERT2)1Feil,
Tg(Tagln-rtTA-cre)1Llw,
Tg(Tal1-cre/ERT)1Jrg,
Tg(Tal1-cre/ERT)42-056Jrg,
Tg(Tas2r143-cre/ERT2)#Soff,
Tg(Tbx18-icre)3Fech,
Tg(Tbx1-cre)1Joe,
Tg(T-cre)1Lwd,
Tg(Tek-cre)12Flv,
Tg(Tek-cre)1Arnd,
Tg(Tek-cre)1Rwng,
Tg(Tek-cre)1Ywa,
Tg(Tek-cre)2352Rwng,
Tg(Tek-cre)5326Sato,
Tg(Tek-cre/ERT2)1Arnd,
Tg(Tek-cre)#Xya,
Tg(Thy1-cre)1Vln,
Tg(Thy1-cre)703Vaw,
Tg(Tie1-cre)9Ref,
Tg(Tnni3-cre/GFP)1Mknn,
Tg(Tnnt2-cre)5Blh,
Tg(Tshb-cre)4Sac,
Tg(Vav1-cre)1Awr,
Tg(Vav1-cre)1Cgp,
Tg(VAV1-cre)1Graf,
Tg(Vav1-cre)8Cgp,
Tg(Vil1-cre)997Gum,
Tg(VIPR2/Hspa1b-cre/ERT2)17Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)1Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)20Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)22Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)23Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)29Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)30Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)32Dlee,
Tg(VIPR2/Hspa1b-cre/ERT2)33Dlee,
Tg(Wfs1-cre/ERT2)3Aibs,
Tg(Wnt11-cre/ERT2)1Jwng,
Tg(Wnt5a-cre/ERT2)#Jwng,
Tg(Wt1-cre)1Jbeb,
Tg(WT1-cre)AG11Dbdr,
Tg(Wt1-cre)#Jbeb,
Tg(Zp3-cre)3Mrt,
Thtm1(cre)Te,
Tnni3em1(dre)Bzsh,
Tnni3tm1(EGFP/cre/ERT2)Wtsi,
Tnnt2em1(dre)Bzsh,
Tp(X)1SoffTg(Myh11-icre/ERT2)1Soff,
Twist2tm1.1(cre)Dor,
Vimem1(cre/ERT2)Bzsh,
Viptm1(cre)Zjh,
Wt1em1(cre/ERT2)Bzsh,
Wt1tm1(EGFP/cre)Wtp,
Wt1tm2(cre/ERT2)Wtp
(less)
|
|
|
Images
|
Drag images to compare to others or to data in the table below. Drag corners to resize images for more detail.
Reset Images
J:291145
Fig. 3
J:291145
Fig. 4
|
|
Recombinase Activity
|
Click heading to re-sort table.
|
|
References
|
|
Analysis Tools