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Rxfp1em1(cre)Ngai - Nervous System
Recombinase Activity Detail
Allele Information
Allele:
Rxfp1em1(cre)Ngai
relaxin/insulin-like family peptide receptor 1; endonuclease-mediated mutation 1, John Ngai 		Driver: Rxfp1
Type: Endonuclease-mediated (Recombinase)
Synonym: Rxfp1-P2A-Cre
Molecular description: The bicistronic Rxfp1-P2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) the gene. The targeting vector, Cas9 endonuclease, and guide-RNA were mixed and injected into the v6.5 mouse embryonic stem cells. Colonies grown from transfected cells were directly screened for successful integration and were aggregated with albino morula and implanted into pseudopregnant females, which produced germ-line-competent chimeric founders. The ampicillin cassette is still present in this allele.
Find mice (IMSR): Mouse Strains: 1 strain available       Cell Lines: 0 lines available
Additional Tissues: Rxfp1em1(cre)Ngai activity also observed in:
endocrine system, head
Tissue Information
Nervous System Other recombinase alleles with activity in Nervous System tissues:
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 1 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory