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Tg(Amigo2-icre/ERT2)1Ehs - Head
Recombinase Activity Detail
Allele Information
Allele:
Tg(Amigo2-icre/ERT2)1Ehs
transgene insertion 1, National Institute of Environmental Health Science 		Driver: Amigo2
Type: Transgenic (Inducible, Recombinase)
Inducer: tamoxifen
Synonym: Amigo2-icreERT2
Molecular description: The BAC clone RP23-288P18 was modified with recombineering techniques so that an icreERT2 is driven by the Amigo2 promotor. The Amigo2 ATG was replaced with the icre ATG preceded with a perfect KOZAK sequence. At the 3 end of the icreERT2 cassette, a synthetic bovine growth hormone (BGH) polyadenylation signal was added after the STOP codon. For the selection of recombined BACs, a flipase-site flanked neomycin resistance gene was incorporated into the targeting fragment following the icreERT2 cassette. Recombined BACs without the neo marker were linearized and purified and microinjected into pronuclei of B6SJLF1 mouse oocytes. Six independent founder mice resulted. Two lines showing adult expression of icreERT2 in CA2 were established. This is line 1 of two lines. This line showed selective expression in CA2 within the hippocampus as well as expression in the fasciola cinerea and hypothalamus.
Find mice (IMSR): Mouse Strains: 0 strains available       Cell Lines: 0 lines available
Additional Tissues: Tg(Amigo2-icre/ERT2)1Ehs activity also observed in:
cardiovascular system, nervous system
Tissue Information
Head Other recombinase alleles with activity in Head tissues:
Images
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J:288826 Fig. 1 J:288826 Fig. 1 S1 J:288826 Fig. 1 S2
Recombinase Activity
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Assays Genotypic Background Result Notes  
References
All for this allele: 3 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory