Cartpt<tm1.2(cre/ERT2)Hhz> - Head Recombinase Activity Detail MGI Mouse

Cartpt^{tm1.2(cre/ERT2)Hhz} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Recombinase Activity | References

Allele Information

Allele:

Cartpt^{tm1.2(cre/ERT2)Hhz}
CART prepropeptide; targeted mutation 1.2, Herbert Herzog

Driver: Cartpt
Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)

Inducer:

tamoxifen

Synonym:

CART^-, CART^cre

Molecular description:

The targeting vector was designed to replace the CART coding exons with the mouse Cartpt cDNA fused to the original CART gene at the initiation codon, which is followed by the insertion of a flippase (Flp) recombinase-recombination target (FRT)-flanked neomycin selection cassette. A cassette carrying the tamoxifen-inducible Cre-recombinase gene followed by an internal ribosome entry site (IRES) that drives the expression of the mCherry red fluorescence reporter gene was inserted. Chimeric offspring were bred with a germline Flp-recombinase mouse line to remove the neomycin cassette. LoxP sites were placed immediately upstream of the initiation codon of the CART and Cre genes respectively, which allows the replacement of the CART gene by Cre. Homozygous mice were then crossed with mice that expressed Cre specifically in the oocytes, resulting in a rearrangement that combines the inducible Cre gene with the endogenous CART promoter.

Find mice (IMSR):

Mouse Strains: 0 strains available Cell Lines: 0 lines available

Additional Tissues:

Cartpt^{tm1.2(cre/ERT2)Hhz} activity also observed in:
nervous system

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

2 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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