Pou4f3<tm1.2(cre)Tcba> - Head Recombinase Activity Detail MGI Mouse

Pou4f3^{tm1.2(cre)Tcba} - Head
Recombinase Activity Detail

Allele Information | Tissue Information | Images | Recombinase Activity | References

Allele Information

Allele:

Pou4f3^{tm1.2(cre)Tcba}
POU domain, class 4, transcription factor 3; targeted mutation 1.2, Tudor Constantin Badea

Driver: Pou4f3
Type: Targeted (Null/knockout, Recombinase)

Synonym:

Brn3c^Cre

Molecular description:

The targeting consists of a FREX cassette (containing the rox12 and roxP target sites for the Dre recombinase in direct orientation, separated by a 68 bp spacer) inserted in the 5' untranslated region (UTR) of the Pou4f3 locus, 65 bp before the initiator codon ATG; three repeats of the SV40 early region transcription terminator inserted in the 3' UTR, 564 bp downstream of the STOP codon; the Cre recombinase cDNA in reverse orientation and a second FREX cassette in reverse orientation; and the Neomycin positive selection cassette (PGKNeo) flanked by FRT (Flp recombinase target) sites. The targeting vector was introduced to 129-derived R1 embryonic stem cells. The neomycin cassette was excised with a germline FLP recombinase. Dre-mediated recombination generated inversion-excision at this locus results in the expression of Cre from the Pou4f3 promoter.

Find mice (IMSR):

Mouse Strains: 0 strains available Cell Lines: 0 lines available

Additional Tissues:

Pou4f3^{tm1.2(cre)Tcba} activity also observed in:
sensory organs

Tissue Information

Head

Other recombinase alleles with activity in Head tissues:

7630403G23Rik^{Tg(Th-cre)1Tmd}, Abcg2^{tm1.1(cre/Esr1*)Jvbe}, Acan^{tm1(cre/ERT2)Crm}, Acsm4^tm1(cre)Hsak ...(more)

Images

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J:322005 Fig. 3

J:322005 Fig. 4

J:322005 Fig. 5

Recombinase Activity

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Assays Genotypic Background Result Notes

References

All for this allele:

1 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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