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Gene Expression Data
Assay Details
Assay
Reference: J:85473 Corbin JG, et al., Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development. 2003 Oct;130(20):4895-906
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2676225
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6 (#PRB-278P; #30130, #901301)
Detection system: Secondary antibody
Assay notes: Not specified in reference whether the antibody used was mouse anti-Pax6 (MGI:2447626) or rabbit anti-Pax6 (MGI:2157328).
Results
Specimen 1A,1C: embryonic day 9.5 (more )
Note: 23 somites. Triple labeled: green - Pax6; red - Nkx2-1 (assay MGI:2676233); blue - Shh (assay MGI:2676237).
Structure Level Pattern Image Note
TS15: telencephalon Present Regionally restricted 1A , 1C Expression of Pax6 and Nkx2.1 forms a boundary in the ventral telencephalon.
Specimen 1D,1F: embryonic day 10.0 (more )
Note: 27 somites. Triple labeled: green - Pax6; red - Nkx2-1 (assay MGI:2676233); blue - Gsx2 (assay MGI:2676238).
Structure Level Pattern Image Note
TS16: telencephalon Present Regionally restricted 1D , 1F Slight gap between Pax6 and Nkx2.1 expression.
Specimen 1G,1H,1I: embryonic day 10.5 (more )
Note: Triple labeled: green - Pax6; red - Nkx2-1 (assay MGI:2676233); blue - Gsx2 (assay MGI:2676238).
Structure Level Pattern Image Note
TS17: telencephalon Present Regionally restricted 1G , 1H , 1I Gap in expression of Pax6 and Nkx2.1 expands.
Specimen 3C: embryonic day 9.5 (more )
Note: 22 somites.
Structure Level Pattern Image Note
TS15: telencephalon Present Regionally restricted 3C Expression of Pax6 and Nkx2.1 form a distinct border.
Specimen 3D: embryonic day 9.5; Nkx2-1tm1Shk/Nkx2-1tm1Shk (more )
Note: 22 somites.
Structure Level Pattern Image Note
TS15: telencephalon Present Regionally restricted 3D Expression does not expand ventrally.
Specimen 6E,6M: embryonic day 12.5 (more )
Note: Double labeled: green - Pax6; red - Rbp1 (assay MGI:2676239).
Structure Level Pattern Image Note
TS20: lateral ganglionic eminence Present Regionally restricted 6M , 6E Expression normally extends just across the cortical sulcus into the dorsal lateral ganglionic eminence.
TS20: telencephalon Present Regionally restricted 6M , 6E Expression normally extends just across the cortical sulcus into the dorsal lateral ganglionic eminence.
Specimen 6F,6N: embryonic day 12.5; Gsx2tm1Ssp/Gsx2tm1Ssp (more )
Note: Double labeled: green - Pax6; red - Rbp1 (assay MGI:2676239).
Structure Level Pattern Image Note
TS20: lateral ganglionic eminence Present Regionally restricted 6N , 6F Expression expands ventrally into the lateral ganglionic eminence.
Specimen 6G,6O: embryonic day 12.5; Nkx2-1tm1Shk/Nkx2-1tm1Shk (more )
Note: Double labeled: green - Pax6; red - Rbp1 (assay MGI:2676239).
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 6O , 6G Expression does not expand into the subpallium.
Specimen 6H,6P: embryonic day 12.5; Gsx2tm1Ssp/Gsx2tm1Ssp, Nkx2-1tm1Shk/Nkx2-1tm1Shk (more )
Note: Double labeled: green - Pax6; red - Rbp1 (assay MGI:2676239).
Structure Level Pattern Image Note
TS20: telencephalon Present Regionally restricted 6H , 6P Significant expansion into ventral-most telencephalon.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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11/12/2024
MGI 6.24 		The Jackson Laboratory