Gene Expression Data
Assay Details
Assay
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Reference:
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J:85473
Corbin JG, et al., Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development. 2003 Oct;130(20):4895-906
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2676238
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Gene symbol:
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Gsx2
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Gene name:
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GS homeobox 2
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Results
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Specimen
1E,1F:
embryonic day 10.0
(more )
Note: 27 somites. Triple labeled: blue - Gsx2; green - Pax6 (assay MGI:2676225); red - Nkx2-1 (assay MGI:2676233).
Specimen
1E,1F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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27 somites. Triple labeled: blue - Gsx2; green - Pax6 (assay MGI:2676225); red - Nkx2-1 (assay MGI:2676233).
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Specimen
1H,1I:
embryonic day 10.5
(more )
Note: Triple labeled: blue - Gsx2; green - Pax6 (assay MGI:2676225); red - Nkx2-1 (assay MGI:2676233).
Specimen
1H,1I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: blue - Gsx2; green - Pax6 (assay MGI:2676225); red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: diencephalon |
Present |
Regionally restricted |
1I
,
1H
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Expressed in gap in expression of Pax6 and Nkx2.1 expands, partially overlapping with Pax6 expression, expanded more ventrally into Nkx2.1-positive domain.
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Specimen
2A:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2A
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In anterior telencephalon, expressed in lateral domain.
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Specimen
2B:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2B:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2B
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At the level of the septum, expression is non-overapping with Nkx2.1 expression.
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Specimen
2C:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2C
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More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain.
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Specimen
2D:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
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Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2D
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More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain.
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Specimen
2E:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 10.5
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2E
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More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain.
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Specimen
2F:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Theiler stage of this specimen assigned by curators from EMAGE.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
36-38 somites. Double labeled: green - Gsx2; red - Nkx2-1 (assay MGI:2676233).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
2F
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At the most posterior level of telencephalon, highly expressed in the region of the presumptive caudal ganglionic eminence.
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Specimen
3E:
embryonic day 9.5
(more )
Note: 26-30 somites.
Specimen
3E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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26-30 somites.
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Specimen
3F:
(close )
Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
|
embryonic day 9.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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26-30 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: telencephalon |
Present |
Regionally restricted |
3F
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Expression in the lateral domain does not expand ventrally into the presumptive medial ganglionic eminence region.
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
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