Gene Expression Data
Assay Details
Assay
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Reference:
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J:65401
Sheth B, et al., Differentiation of the epithelial apical junctional complex during mouse preimplantation development: a role for rab13 in the early maturation of the tight junction. Mech Dev. 2000 Oct;97(1-2):93-104
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3044899
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Gene symbol:
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Ctnna1
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Gene name:
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catenin alpha 1
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Antibody:
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anti-alpha catenin
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Detection system:
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Secondary antibody coupled to other - see notes
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Assay notes:
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Secondary antibody was labeled with either TRITC or Alexa 546. Dual labeling confocal microscopy with Tjp1 as shown in assay: MGI:3044895.
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Results
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Specimen
1B,C,D:
embryonic day 2.5
(more )
Note: Compacted 8-cell embryo. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
Specimen
1B,C,D:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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Compacted 8-cell embryo. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
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Structure |
Level |
Pattern |
Image |
Note |
TS3: compacted morula |
Present |
Regionally restricted |
1B
,
1D
,
1C
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Expression is within the cell contact region.
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Specimen
1F,G,H,I:
embryonic day 3.0
(more )
Note: 16-cell embryo. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
Specimen
1F,G,H,I:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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16-cell embryo. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Regionally restricted |
1I
,
1G
,
1H
,
1F
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Expression is within the cell contact region.
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Specimen
1K,L,M:
embryonic day 3.5
(more )
Note: Early blastocyst. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
Specimen
1K,L,M:
(close )
Genetic Background:
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MF1
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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Formaldehyde
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Note:
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Early blastocyst. Double labeled: red - Ctnna1; green - Tjp1 (assay MGI:3044895).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
Regionally restricted |
1L
,
1M
,
1K
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Expression is within the lateral membrane cell contact region.
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