Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:84752
Hartfuss E, et al., Reelin signaling directly affects radial glia morphology and biochemical maturation. Development. 2003 Oct;130(19):4597-609
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3514042
|
Gene symbol:
|
Nes
|
Gene name:
|
nestin
|
Antibody:
|
clone RC2
|
Detection system:
|
Secondary antibody coupled to rhodamine
|
|
Results
|
Specimen
1A,A':
embryonic day 16.5
(more )
Note: Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
Specimen
1A,A':
(close )
Genetic Background:
|
involves: C3HeB/FeJ * C57BL/6J
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex |
Present |
Regionally restricted |
1A'
,
1A
|
Expression is present in the radial glia.
|
Specimen
1B,B':
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
Specimen
1B,B':
(close )
Genetic Background:
|
involves: C3HeB/FeJ * C57BL/6J
|
Mutant Allele(s):
|
Relnrl/Relnrl
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex |
Present |
Regionally restricted |
1B
,
1B'
|
Expression is present in the radial glia; expression levels are similar to those seen in wild type animals.
|
Specimen
1C,C':
embryonic day 16.5
(more )
Note: Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
Specimen
1C,C':
(close )
Genetic Background:
|
involves: C3HeB/FeJ * C57BL/6J
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: ganglionic eminence |
Present |
Regionally restricted |
1C
,
1C'
|
Expression is present in the radial glia of the ganglionic eminence.
|
Specimen
1D,D':
embryonic day 16.5; Relnrl/ Relnrl
(more )
Note: Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
Specimen
1D,D':
(close )
Genetic Background:
|
involves: C3HeB/FeJ * C57BL/6J
|
Mutant Allele(s):
|
Relnrl/Relnrl
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Vibratome section. Double labeled: red - Nes; green - Fabp7 (BLBP; assay MGI:3514043).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: ganglionic eminence |
Present |
Regionally restricted |
1D'
,
1D
|
Expression is present in the radial glia of the ganglionic eminence; expression levels are similar to those seen in wild type animals.
|
|