Gene Expression Data
Assay Details
Assay
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Reference:
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J:97065
Asayesh A, et al., Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments. Dev Dyn. 2005 Apr;232(4):1105-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3580818
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Gene symbol:
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Adam10
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Gene name:
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a disintegrin and metallopeptidase domain 10
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Antibody:
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anti-ADAM10
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Detection system:
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Secondary antibody
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Assay notes:
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The images were confocal images.
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Results
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Specimen
1D, 1J:
embryonic day 10.0
(more )
Note: Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
Specimen
1D, 1J:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: pancreas primordium |
Present |
|
1J
,
1D
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Expression was detected in the dorsal and ventral pancreatic buds as well as in the intervening gut endoderm. Expression was absent or near absent in the pancreas-associated mesenchyme and in a substantial number of cells that coexpressed glucagon.
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Specimen
Table 1:
embryonic day 9.5
(more )
Specimen
Table 1:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: pancreas primordium |
Weak |
Regionally restricted |
|
Expression is low or near absent in the glucagon expressing pancreatic epithelium; it is absent or near absent in the pancreas-associated mesenchyme.
|
Specimen
2J:
embryonic day 12.5
(more )
Note: Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
Specimen
2J:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Weak |
Regionally restricted |
2J
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
2K:
embryonic day 15.5
(more )
Note: Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
Specimen
2K:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Weak |
Regionally restricted |
2K
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
2L:
embryonic day 17.5
(more )
Note: Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
Specimen
2L:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: pancreas |
Present |
Regionally restricted |
2L
|
Expression was prominent in glucagon-expressing cells, indicating an up-regulation in the endocrine compartment.
|
Specimen
2M:
embryonic day 12.5
(more )
Note: Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
2M:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Weak |
Regionally restricted |
2M
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
2N:
embryonic day 15.5
(more )
Note: Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
2N:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Weak |
Regionally restricted |
2N
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
2O:
embryonic day 17.5
(more )
Note: Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
2O:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: pancreas |
Present |
Regionally restricted |
2O
|
Expression was prominent in insulin-expressing cells, indicating an up-regulation in the endocrine compartment.
|
Specimen
Table 1:
embryonic day 14.5
(more )
Specimen
Table 1:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
Weak |
Regionally restricted |
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
Table 1:
embryonic day 16.5
(more )
Specimen
Table 1:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: pancreas |
Weak |
Regionally restricted |
|
Expression was low or near absent in the pancreatic endocrine compartment.
|
Specimen
3D:
postnatal day 1
(more )
Note: Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
Specimen
3D:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Ins1 (assay MGI:3580822) and Ins2 (assay MGI:3580823).
|
Specimen
3E:
postnatal day 1
(more )
Note: Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
Specimen
3E:
(close )
Genetic Background:
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involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Gcg (assay MGI:3580821).
|
Specimen
3F:
postnatal day 1
(more )
Note: Double labeled: red - Adam10; green - Sst (assay MGI:3580819).
Specimen
3F:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Sst (assay MGI:3580819).
|
Specimen
4G:
postnatal week 8
(more )
Specimen
4G:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal week 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: pancreas |
Present |
Regionally restricted |
4G
|
Expression was detected throughout the epithelium.
|
Specimen
5D, 5F:
postnatal adult
(more )
Note: Double labeled: red - Adam10; green - Cdh1 (assay MGI:3580832).
Specimen
5D, 5F:
(close )
Genetic Background:
|
involves: C57BL * CBA
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
Double labeled: red - Adam10; green - Cdh1 (assay MGI:3580832).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: pancreas |
Present |
|
5F
,
5D
|
Expression was predominantly localized to the outer cell membrane in both the endocrine and exocrine compartment. Expression colocalized with Cdh1 (E-cadherin).
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Specimen
6D, 6F:
embryonic day 15.5
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6D, 6F:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This specimen was also stained for DBA-lectin (green).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas |
Present |
Regionally restricted |
6D
,
6F
|
Expression was detected in pancreatic ductal cells.
|
Specimen
6M, 6O:
postnatal week 8
(more )
Note: This specimen was also stained for DBA-lectin (green).
Specimen
6M, 6O:
(close )
Genetic Background:
|
involves: C57BL * CBA
|
Age:
|
postnatal week 8
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
This specimen was also stained for DBA-lectin (green).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: pancreatic duct |
Present |
Regionally restricted |
6O
,
6M
|
Expression was detected in the ductal epithelium.
|
|