Gene Expression Data
Assay Details
Assay
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Reference:
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J:86170
Menke DB, et al., Sexual differentiation of germ cells in XX mouse gonads occurs in an anterior-to-posterior wave. Dev Biol. 2003 Oct 15;262(2):303-12
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3652412
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Gene symbol:
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Aard
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Gene name:
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alanine and arginine rich domain containing protein
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Probe:
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Aard cDNA2
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 14.5
(more )
Note: XYZal Mildly feminized.
Specimen
6A:
(close )
Genetic Background:
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involves: C57BL/6 * ZALENDE/Ei
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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XYZal Mildly feminized.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: gonad |
Present |
Regionally restricted |
6A
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Expression was confined to masculinized regions of mildly feminized ovotestes.
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Specimen
6B:
embryonic day 14.5
(more )
Note: XYZal Severely feminized.
Specimen
6B:
(close )
Genetic Background:
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involves: C57BL/6 * ZALENDE/Ei
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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XYZal Severely feminized.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: gonad |
Present |
Regionally restricted |
6B
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Expression was confined to masculinized regions of severely feminized ovotestes.
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Specimen
6C:
embryonic day 14.5
(more )
Note: XYZal Sex reversed.
Specimen
6C:
(close )
Genetic Background:
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involves: C57BL/6 * ZALENDE/Ei
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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XYZal Sex reversed.
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Structure |
Level |
Pattern |
Image |
Note |
TS22: gonad |
Absent |
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6C
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Expression was absent from fully feminized sex reversed ovaries.
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Specimen
7D:
embryonic day 14.5
(more )
Note: XYZal Mildly feminized. Double labeled: orange/brown - Aard; purple - Stra8 (assay MGI:3652418).
Specimen
7D:
(close )
Genetic Background:
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involves: C57BL/6 * ZALENDE/Ei
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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XYZal Mildly feminized. Double labeled: orange/brown - Aard; purple - Stra8 (assay MGI:3652418).
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Structure |
Level |
Pattern |
Image |
Note |
TS22: gonad |
Present |
Regionally restricted |
7D
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Expression was detected in testicular cords directly adjacent to feminized regions that express Stra8.
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