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Gene Expression Data
Assay Details
Assay
Reference: J:114758 Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3716150
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Assay notes: Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
Results
Specimen 5C +/+ medial Tuj1: embryonic day 15.5 (more )
Note: Triple labeled: red - Tubb3; green - Ebf1 (assay MGI:3716156); blue - DAPI.
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 5C +/+ medial Merge , 5C +/+ medial Tuj1

Specimen 5C nur12 medial Tuj1: embryonic day 15.5; Zfp423nur12/Zfp423nur12 (more )
Note: Triple labeled: red - Tubb3; green - Ebf1 (assay MGI:3716156); blue - DAPI.
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 5C nur12 medial Tuj1 , 5C nur12 medial Merge Expression in the most superficial layers of the ventricular zone.

Specimen 5C +/+ lateral Tuj1: embryonic day 15.5 (more )
Note: Triple labeled: red - Tubb3; green - Ebf1 (assay MGI:3716156); blue - DAPI.
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 5C +/+ lateral Merge , 5C +/+ lateral Tuj1

Specimen 5C nur12 lateral Tuj1: embryonic day 15.5; Zfp423nur12/Zfp423nur12 (more )
Note: Triple labeled: red - Tubb3; green - Ebf1 (assay MGI:3716156); blue - DAPI.
Structure Level Pattern Image Note
TS23: cerebellum Present Regionally restricted 5C nur12 lateral Tuj1 , 5C nur12 lateral Merge Expression in the most superficial layers of the ventricular zone.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory