Probe:	IMAGE clone 3369723
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	5d	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	5e	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	5g	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	5h	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S4_y	Expression is present in the anterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	S4_z	Expression is present in the anterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S4_aa	Expression is present in the anterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	S4_bb	Expression is present in the dorsomedial hypothalamic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Very strong	Non-Uniform	Lhx8-Shh E11.5-sagittal , Lhx8-Shh E11.5-coronal	There is very strong staining in the MZ of the ventral POA & anterior ID. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, dorsal POA, PVH, VMH & ARH nuclei, the AVH, posterior ID, premam, mam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer	Ambiguous		Lhx8-Shh E11.5-sagittal , Lhx8-Shh E11.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer	Very strong	Non-Uniform	Lhx8-Shh E11.5-sagittal , Lhx8-Shh E11.5-coronal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex
TS19: telencephalon ventricular layer	Ambiguous		Lhx8-Shh E11.5-sagittal , Lhx8-Shh E11.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Lhx8-Shh E12.5-sagittal , Lhx8-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Very strong	Non-Uniform	Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx8-Shh E12.5-coronal , Lhx8-Shh E12.5-sagittal	There is very strong staining in the mantle zone of the dorsal POA and anterior ID. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei, the AVH and posterior ID.
TS20: hypothalamus ventricular layer	Ambiguous		Lhx8-Shh E12.5-sagittal , Lhx8-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Ambiguous		Lhx8-Shh E12.5-sagittal , Lhx8-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Lhx8-Shh E12.5-sagittal , Lhx8-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Lhx8-Shh E14.5-sagittal , Lhx8-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Very strong	Non-Uniform	Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	There is very strong staining in the mantle zone of the dorsal POA and the anterior ID. Staining is either absent or ubiquitous in the mantle zone of the ventral POAs the PVH, VMH and ARH nuclei, the AVH and posterior ID.
TS22: hypothalamus ventricular layer	Ambiguous		Lhx8-Shh E14.5-sagittal , Lhx8-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Ambiguous		Lhx8-Shh E14.5-coronal , Lhx8-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Lhx8-Shh E14.5-sagittal , Lhx8-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Lhx8-Shh E16.5-coronal , Lhx8-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Very strong	Non-Uniform	Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx8-Shh E16.5-coronal , Lhx8-Shh E16.5-sagittal	There is very strong staining in the mantle zone of the dorsal POA and DMH nucleus. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, SCH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Ambiguous		Lhx8-Shh E16.5-coronal , Lhx8-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Lhx8-Shh E16.5-sagittal , Lhx8-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.