Probe:	UI-M-BH3-awh-f-01-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	3h	Expression is present in the prethalamus.
TS20: hypothalamus	Present	Regionally restricted	3h	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	6g	Expression is present in the intrahypothalamic diagonal.
TS20: diencephalon	Present	Regionally restricted	6g	Expression is present in the tuberomammillary terminal.
TS20: thalamus	Present	Regionally restricted	6g	Expression is present in the posterior prethalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	6h	Expression is present in the posterior prethalamus.
TS20: hypothalamus	Absent		6h	There is no expression in the intrahypothalamic diagonal or tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Strong	Non-Uniform	Arx-Shh E11.5-coronal-1 , Arx-Shh E11.5-sagittal	Staining is strong in the MZ of anterior & posterior IDs & TT, moderate in the dorsal POA, weak & scattered in the dorsal thalamus & either absent or ubiquitous in the prethalamus, ventral POA, PVH, VMH & ARH nuclei, AVH & premam, mam & supramam zones.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Arx-Shh E11.5-coronal-1 , Arx-Shh E11.5-sagittal	There is strong staining in the VZ of the prethalamus. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam, mam & supramam zones & the TT.
TS19: telencephalon mantle layer	Strong	Non-Uniform	Arx-Shh E11.5-coronal-1 , Arx-Shh E11.5-sagittal	There is strong staining in the mantle zone of the ganglionic eminences and weak staining in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Weak	Non-Uniform	Arx-Shh E11.5-sagittal , Arx-Shh E11.5-coronal-1	There is weak staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Moderate		Arx-Shh E12.5-coronal , Arx-Shh E12.5-sagittal	There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Moderate		Arx-Shh E12.5-coronal , Arx-Shh E12.5-sagittal	There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Moderate		Arx-Shh E12.5-coronal , Arx-Shh E12.5-sagittal	There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Strong	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS20: diencephalon ventricular layer	Weak	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is weak, scattered staining in the ventricular zone of the TT. Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS20: ganglionic eminence	Very strong	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is very strong staining in the mantle zone of the ganglionic eminences and weak, scattered staining in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is strong staining in the mantle zone of the dorsal and ventral POAs. Staining is either absent or ubiquitous in the mantle zone of the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Arx-Shh E12.5-coronal , Arx-Shh E12.5-sagittal	There is strong staining in the ventricular zone of the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and the AVH.
TS20: thalamus mantle layer	Very strong	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS20: thalamus ventricular layer	Weak	Non-Uniform	Arx-Shh E12.5-sagittal , Arx-Shh E12.5-coronal	There is weak, scattered staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Arx-Shh E14.5-sagittal , Arx-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Moderate		Arx-Shh E14.5-coronal , Arx-Shh E14.5-sagittal	There is moderate, scattered staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Arx-Shh E14.5-coronal , Arx-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Strong	Non-Uniform	Arx-Shh E14.5-sagittal , Arx-Shh E14.5-coronal	There is strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS22: diencephalon ventricular layer	Ambiguous		Arx-Shh E14.5-coronal , Arx-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Very strong	Non-Uniform	Arx-Shh E14.5-sagittal , Arx-Shh E14.5-coronal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Strong	Non-Uniform	Arx-Shh E14.5-coronal , Arx-Shh E14.5-sagittal	There is strong staining in the mantle zone of the dorsal and ventral POAs and strong, scattered staining in the AVH and anterior ID. Staining is either absent or ubiquitous in the mantle zone of the PVH, VMH and ARH nuclei and the posterior ID.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Arx-Shh E14.5-sagittal , Arx-Shh E14.5-coronal	There is strong, scattered staining in the ventricular zone of the posterior ID. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior ID.
TS22: thalamus mantle layer	Ambiguous		Arx-Shh E14.5-coronal , Arx-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Arx-Shh E14.5-sagittal , Arx-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong	Scattered	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is strong, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Very strong	Scattered	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is very strong, scattered staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Strong	Scattered	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is strong, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Strong	Non-Uniform	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones.
TS24: diencephalon ventricular layer	Ambiguous		Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Ambiguous		Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	Staining is very strong in the MZ of the dorsal POA, strong in the ventral POA & ARH nucleus, strong & scattered in the AVH & the DAH & moderate & scattered in the PH. Staining is either absent or ubiquitous in the MZ of the PVH, SCH, DMH & VMH nuclei.
TS24: hypothalamus ventricular layer	Moderate	Non-Uniform	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is moderate, scattered staining in the ventricular zone of the DMH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Very strong	Non-Uniform	Arx-Shh E16.5-coronal , Arx-Shh E16.5-sagittal	There is very strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS24: thalamus ventricular layer	Ambiguous		Arx-Shh E16.5-sagittal , Arx-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.