Probe:	MGC clone 85996
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	3e	Expression is present in the intrahypothalamic diagonal.
TS20: diencephalon	Present	Regionally restricted	3e	Expression is present in the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	5a	Expression is present in the posterior portion of the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	5b	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	6m	Expression is present in the intrahypothalamic diagonal.
TS20: diencephalon	Present	Regionally restricted	6m	Expression is present in the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Absent		6n	There is no expression in the intrahypothalamic diagonal.
TS20: diencephalon	Absent		6n	There is no expression in the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S4_m	Expression is present in the posterior intrahypothalamic diagonal and the tuberomammillary terminus.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	S4_n	Expression is present in the tuberomammillary terminal.
TS20: hypothalamus	Present	Regionally restricted	S4_n	Expression is present in the posterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	S4_o	Expression is present in the tuberomammillary terminal.
TS22: hypothalamus	Present	Regionally restricted	S4_o	Expression is present in the posterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present	Regionally restricted	S4_p	Expression is present in the tuberomammillary terminal.
TS24: hypothalamus	Present	Regionally restricted	S4_p	Expression is present in the posterior intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Very strong	Non-Uniform	Lhx6-Shh E11.5-coronal , Lhx6-Shh E11.5-sagittal	There is very strong staining in the MZ of posterior ID & the TT. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior ID & premam, mam & supramam zones.
TS19: diencephalon lateral wall ventricular layer	Ambiguous		Lhx6-Shh E11.5-coronal , Lhx6-Shh E11.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer	Very strong	Non-Uniform	Lhx6-Shh E11.5-sagittal , Lhx6-Shh E11.5-coronal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Very strong	Non-Uniform	Lhx6-Shh E11.5-coronal , Lhx6-Shh E11.5-sagittal	There is very strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Very strong	Non-Uniform	Lhx6-Shh E12.5-coronal , Lhx6-Shh E12.5-sagittal	There is very strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS20: diencephalon ventricular layer	Ambiguous		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Very strong		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	There is very strong staining in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx6-Shh E12.5-coronal , Lhx6-Shh E12.5-sagittal	There is very strong staining in the mantle zone of the posterior ID and strong staining in the dorsal POA. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei, the AVH and anterior ID.
TS20: hypothalamus ventricular layer	Ambiguous		Lhx6-Shh E12.5-coronal , Lhx6-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Ambiguous		Lhx6-Shh E12.5-sagittal , Lhx6-Shh E12.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Lhx6-Shh E12.5-coronal , Lhx6-Shh E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Lhx6-Shh E14.5-sagittal , Lhx6-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Very strong	Non-Uniform	Lhx6-Shh E14.5-sagittal , Lhx6-Shh E14.5-coronal	There is very strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS22: diencephalon ventricular layer	Ambiguous		Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Very strong		Lhx6-Shh E14.5-sagittal , Lhx6-Shh E14.5-coronal	There is very strong staining in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx6-Shh E14.5-sagittal , Lhx6-Shh E14.5-coronal	There is very strong staining in the mantle zone of the dorsal POA and posterior ID. Staining is either absent or ubiquitous in the mantle zone of the ventral POAs the PVH, VMH and ARH nuclei, the AVH and anterior ID.
TS22: hypothalamus ventricular layer	Very strong	Non-Uniform	Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	There is very strong staining in the ventricular zone of the dorsal POA. Staining is either absent or ubiquitous in the ventricular zone of the ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Ambiguous		Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Lhx6-Shh E14.5-coronal , Lhx6-Shh E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Moderate		Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Moderate		Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Very strong	Non-Uniform	Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is very strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zone.
TS24: diencephalon ventricular layer	Ambiguous		Lhx6-Shh E16.5-coronal , Lhx6-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Very strong		Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is very strong staining in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is very strong staining in the mantle zone of the dorsal POA and the PH. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH and the DAH.
TS24: hypothalamus ventricular layer	Very strong	Non-Uniform	Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal POA. Staining is either absent or ubiquitous in the ventricular zone of the ventral POA, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Lhx6-Shh E16.5-sagittal , Lhx6-Shh E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Lhx6-Shh E16.5-coronal , Lhx6-Shh E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.