Results
|
Specimen
4a:
embryonic day 12.5
(more )
Note: Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
Specimen
4a:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
4a
|
Expression is present in the mammillary nucleus.
|
Specimen
4b:
embryonic day 16.5
(more )
Note: Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
Specimen
4b:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Lhx6 (assay MGI:4452075).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
4b
|
Expression is present in the mammillary nucleus.
|
Specimen
6c:
embryonic day 12.5
(more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Specimen
6c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
6c
|
Expression is present in the mammillary epithelium.
|
Specimen
6d:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
Shhtm2Amc/Shhtm2Amc Tg(Nkx2-1-cre)2Sand/0
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant. Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
6d
|
Expression is present in the mammillary epithelium.
|
Specimen
S4e:
embryonic day 11.5
(more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Specimen
S4e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
S4_e
|
Expression is present in the mammillary nucleus.
|
Specimen
S4f:
embryonic day 12.5
(more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Specimen
S4f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
S4_f
|
Expression is present in the mammillary nucleus.
|
Specimen
S4g:
embryonic day 14.5
(more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Specimen
S4g:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
S4_g
|
Expression is present in the mammillary nucleus.
|
Specimen
S4h:
embryonic day 16.5
(more )
Note: Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
Specimen
S4h:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1; brown - Shh (assay MGI:4452154).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
S4_h
|
Expression is present in the mammillary nucleus.
|
Specimen
Foxb1 - E11.5:
embryonic day 11.5
(more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Specimen
Foxb1 - E11.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon lateral wall mantle layer |
Very strong |
Non-Uniform |
FOXB1(D)+SHH(F)-E11.5-coronal
,
FOXB1(D)+SHH(F)-E11.5-sagittal
|
There is very strong staining in the MZ of the mam zone. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam & supramam zones & TT.
|
TS19: diencephalon lateral wall ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E11.5-sagittal
,
FOXB1(D)+SHH(F)-E11.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
|
TS19: telencephalon mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E11.5-sagittal
,
FOXB1(D)+SHH(F)-E11.5-coronal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
|
TS19: telencephalon ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E11.5-sagittal
,
FOXB1(D)+SHH(F)-E11.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.
|
Specimen
Foxb1 - E12.5:
embryonic day 12.5
(more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Specimen
Foxb1 - E12.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex intermediate zone |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-coronal
,
FOXB1(D)+SHH(F)-E12.5-sagittal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
|
TS20: cerebral cortex ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-coronal
,
FOXB1(D)+SHH(F)-E12.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
|
TS20: cortical plate |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
|
TS20: diencephalon mantle layer |
Very strong |
Non-Uniform |
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
|
TS20: diencephalon ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
|
TS20: ganglionic eminence |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
|
TS20: hypothalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-coronal
,
FOXB1(D)+SHH(F)-E12.5-sagittal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
|
TS20: hypothalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
|
TS20: thalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
|
TS20: thalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E12.5-sagittal
,
FOXB1(D)+SHH(F)-E12.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
|
Specimen
Foxb1 - E14.5:
embryonic day 14.5
(more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Specimen
Foxb1 - E14.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex intermediate zone |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-coronal
,
FOXB1(D)+SHH(F)-E14.5-sagittal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
|
TS22: cerebral cortex ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-coronal
,
FOXB1(D)+SHH(F)-E14.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
|
TS22: cortical plate |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-coronal
,
FOXB1(D)+SHH(F)-E14.5-sagittal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
|
TS22: diencephalon mantle layer |
Very strong |
Non-Uniform |
FOXB1(D)+SHH(F)-E14.5-sagittal
,
FOXB1(D)+SHH(F)-E14.5-coronal
|
There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
|
TS22: diencephalon ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-coronal
,
FOXB1(D)+SHH(F)-E14.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
|
TS22: ganglionic eminence |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-coronal
,
FOXB1(D)+SHH(F)-E14.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
|
TS22: hypothalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-sagittal
,
FOXB1(D)+SHH(F)-E14.5-coronal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
|
TS22: hypothalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-sagittal
,
FOXB1(D)+SHH(F)-E14.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
|
TS22: thalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-sagittal
,
FOXB1(D)+SHH(F)-E14.5-coronal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
|
TS22: thalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E14.5-sagittal
,
FOXB1(D)+SHH(F)-E14.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
|
Specimen
Foxb1 - E16.5:
embryonic day 16.5
(more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Specimen
Foxb1 - E16.5:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex intermediate zone |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-coronal
,
FOXB1(D)+SHH(F)-E16.5-sagittal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
|
TS24: cerebral cortex ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
|
TS24: cortical plate |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
|
TS24: diencephalon mantle layer |
Very strong |
Non-Uniform |
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones.
|
TS24: diencephalon ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-coronal
,
FOXB1(D)+SHH(F)-E16.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
|
TS24: ganglionic eminence |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
|
TS24: hypothalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
|
TS24: hypothalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-coronal
,
FOXB1(D)+SHH(F)-E16.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
|
TS24: thalamus mantle layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-sagittal
,
FOXB1(D)+SHH(F)-E16.5-coronal
|
Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
|
TS24: thalamus ventricular layer |
Ambiguous |
|
FOXB1(D)+SHH(F)-E16.5-coronal
,
FOXB1(D)+SHH(F)-E16.5-sagittal
|
Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
|
|