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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450860
Gene symbol: Fst
Gene name: follistatin
Probe: IMAGE clone 5366191
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen S8ee: embryonic day 11.5 (more )
Note: Double labeled: blue - Fst; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_ee Areas of expression include the intrahypothalamic diagonal.
Specimen S8ff: embryonic day 11.5 (more )
Note: Double labeled: blue - Fst; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_ff Areas of expression include the intrahypothalamic diagonal.
Specimen S8gg: embryonic day 11.5 (more )
Note: Double labeled: blue - Fst; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_gg Areas of expression include the intrahypothalamic diagonal.
Specimen S8hh: embryonic day 11.5 (more )
Note: Double labeled: blue - Fst; brown - Shh (assay MGI:4452154).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted S8_hh Areas of expression include the intrahypothalamic diagonal.
Specimen Fst - E11.5: embryonic day 11.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Ambiguous Fst-Shh E11.5-sagittal-2 , Fst-Shh E11.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Moderate Non-Uniform Fst-Shh E11.5-sagittal-2 , Fst-Shh E11.5-coronal Staining is moderate in the VZ of the dorsal thalamus, prethalamus, dorsal POA, PVH nucleus, anterior & posterior IDs, premam zone & TT. Staining is either absent or ubiquitous in the VZ of the ventral POA, AVH, VMH & ARH nuclei & mam & supramam zones.
TS19: telencephalon mantle layer Ambiguous Fst-Shh E11.5-sagittal-2 , Fst-Shh E11.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Ambiguous Fst-Shh E11.5-sagittal-2 , Fst-Shh E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.
Specimen Fst - E12.5: embryonic day 12.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Fst-Shh E12.5-coronal , Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-sagittal , Fst-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-coronal , Fst-Shh E12.5-sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-sagittal , Fst-Shh E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Moderate Non-Uniform Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-sagittal , Fst-Shh E12.5-coronal There is moderate staining in the ventricular zone of the premam zone and the TT. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS20: ganglionic eminence Ambiguous Fst-Shh E12.5-sagittal , Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-sagittal , Fst-Shh E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Weak Non-Uniform Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-coronal , Fst-Shh E12.5-sagittal There is weak staining in the ventricular zone of the dorsal and ventral POAs and the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the PVH, VMH and ARH nuclei and the AVH.
TS20: thalamus mantle layer Ambiguous Fst-Shh E12.5-sagittal-2 , Fst-Shh E12.5-coronal , Fst-Shh E12.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Moderate Fst-Shh E12.5-sagittal , Fst-Shh E12.5-coronal , Fst-Shh E12.5-sagittal-2 There is moderate staining in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Fst - E14.5: embryonic day 14.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal , Fst-Shh E14.5-sagittal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-sagittal , Fst-Shh E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Fst-Shh E14.5-sagittal , Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous Fst-Shh E14.5-sagittal , Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Moderate Non-Uniform Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-sagittal , Fst-Shh E14.5-coronal There is moderate staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones and the TT.
TS22: ganglionic eminence Ambiguous Fst-Shh E14.5-sagittal , Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous Fst-Shh E14.5-sagittal , Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal-2 Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous Fst-Shh E14.5-coronal , Fst-Shh E14.5-sagittal-2 , Fst-Shh E14.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
Specimen Fst - E16.5: embryonic day 16.5 (more )
Note: Specimen was also hybridized with a fluorescein-labeled Shh probe (MGI:2110655; detected with TBT/BCIP). The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-sagittal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal-2 Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-coronal , Fst-Shh E16.5-sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous Fst-Shh E16.5-sagittal , Fst-Shh E16.5-sagittal-2 , Fst-Shh E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.
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11/19/2024
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