Probe:	UI-M-AJ1-ahc-g-03-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_p	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_q	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_r	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S8_s	Expression is present throughout the prethalamus and in a portion of the anteriodorsal hypothalamus that includes the paraventricular hypothalamic nucleus and the intrahypothalamic diagonal; it also extends along the tuberomammillary terminal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Weak	Non-Uniform	Wnt7a-Shh E11.5_coronal , Wnt7a-Shh E11.5_sagittal	There is weak staining in the MZ of the dorsal POA. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, prethalamus, ventral POA, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam, mam & supramam zones & the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E11.5_sagittal , Wnt7a-Shh E11.5_coronal	Staining is strong in the VZ of the prethalamus, PVH nucleus, posterior ID, premam zone & TT, weak in the thalamus & dorsal POA & either absent or ubiquitous in the ventral POA, AVH, anterior ID, VMH & ARH nuclei & mam & supramam zones.
TS19: telencephalon mantle layer	Weak	Non-Uniform	Wnt7a-Shh E11.5_sagittal , Wnt7a-Shh E11.5_coronal	There is weak, scattered staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E11.5_coronal , Wnt7a-Shh E11.5_sagittal	There is strong staining in the ventricular zone of the cerebral cortex and weak, scattered staining in the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Wnt7a-Shh E12.5_sagittal , Wnt7a-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Strong		Wnt7a-Shh E12.5_sagittal , Wnt7a-Shh E12.5_coronal	There is strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Wnt7a-Shh E12.5_sagittal , Wnt7a-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Wnt7a-Shh E12.5_coronal , Wnt7a-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E12.5_coronal , Wnt7a-Shh E12.5_sagittal	There is strong staining in the ventricular zone of the premam zone and the TT and moderate staining in the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS20: ganglionic eminence	Strong	Non-Uniform	Wnt7a-Shh E12.5_coronal , Wnt7a-Shh E12.5_sagittal	There is strong staining in the mantle zone of the ganglionic eminences and weak, scattered staining in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Wnt7a-Shh E12.5_coronal , Wnt7a-Shh E12.5_sagittal	There is strong staining in the mantle zone of the anterior and posterior IDs and moderate staining in the dorsal POA. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei and the AVH.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E12.5_sagittal , Wnt7a-Shh E12.5_coronal	There is strong staining in the ventricular zone of the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and the AVH.
TS20: thalamus mantle layer	Ambiguous		Wnt7a-Shh E12.5_coronal , Wnt7a-Shh E12.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Strong		Wnt7a-Shh E12.5_sagittal , Wnt7a-Shh E12.5_coronal	There is strong staining in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Very strong		Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Strong	Non-Uniform	Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is strong staining in the mantle zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the mantle zone of the supramam zone.
TS22: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is strong staining in the ventricular zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS22: ganglionic eminence	Strong		Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is strong staining in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Strong	Non-Uniform	Wnt7a-Shh E14.5_sagittal , Wnt7a-Shh E14.5_coronal	There is strong staining in the mantle zone of the dorsal POA and weak staining in the anterior and posterior IDs. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei and the AVH.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is strong staining in the ventricular zone of the ventral POA, the VMH nucleus, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal POA and the PVH and ARH nuclei.
TS22: thalamus mantle layer	Ambiguous		Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E14.5_coronal , Wnt7a-Shh E14.5_sagittal	There is strong staining in the ventricular zone of the prethalamus and moderate staining in the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Strong		Wnt7a-Shh E16.5_coronal , Wnt7a-Shh E16.5_sagittal	There is strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Wnt7a-Shh E16.5_coronal , Wnt7a-Shh E16.5_sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Strong	Non-Uniform	Wnt7a-Shh E16.5_coronal , Wnt7a-Shh E16.5_sagittal	There is strong staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones.
TS24: diencephalon ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E16.5_coronal , Wnt7a-Shh E16.5_sagittal	There is strong staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the mam and supramam zones.
TS24: ganglionic eminence	Moderate		Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	There is moderate staining in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Weak	Non-Uniform	Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	There is weak staining in the mantle zone of the DAH and the PH and weak, scattered staining in the DMH nucleus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, VMH and ARH nuclei and the AVH.
TS24: hypothalamus ventricular layer	Strong	Non-Uniform	Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	There is strong staining in the ventricular zone of the dorsal and ventral POAs, the DMH nucleus, the AVH, the DAH and the PH. Staining is either absent or ubiquitous in the ventricular zone of the PVH, SCH, VMH and ARH nuclei.
TS24: thalamus mantle layer	Ambiguous		Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Strong		Wnt7a-Shh E16.5_sagittal , Wnt7a-Shh E16.5_coronal	There is strong staining in the ventricular zone of the dorsal thalamus and prethalamus.