Probe:	IMAGE clone 6850036
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		4i

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Present		4j

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	5j	Expression is present in the suprachiasmatic nucleus and in a region immediately posterodorsal to the nucleus that probably represents the ventral subparaventricular zone.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	5k	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	5l	Expression is present in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	6k	Expression is present in the intrahypothalamic diagonal.
TS20: thalamus	Present	Regionally restricted	6k	Expression is present in the anterior and posterior prethalamus.
TS20: diencephalon	Present	Regionally restricted	6k	Expression is present in the mammillary hypothalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	6l	Expression is present in the anterior and posterior prethalamus.
TS20: diencephalon	Present	Regionally restricted	6l	Expression is present in the mammillary hypothalamus, as well as in a prominent anterior-located region that is dorsal to the optic recess and resides in the preoptic neuroepithelium.
TS20: hypothalamus	Absent		6l	There is no expression in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		S7_e	This annotation was inferred from the data presented in Supp. Table 4.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	S7_f	Expression is present in the developing suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S7_g	Expression is present in the developing suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	S7_h	Expression is present in the developing suprachiasmatic nucleus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Strong	Non-Uniform	LHX1(D)+SHH(F)-E11.5-coronal , LHX1(D)+SHH(F)-E11.5-sagittal	Staining is strong in the MZ of the prethalamus & mam zone & weak in the anterior ID. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, AVH, posterior ID, premam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E11.5-coronal , LHX1(D)+SHH(F)-E11.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer	Ambiguous		LHX1(D)+SHH(F)-E11.5-coronal , LHX1(D)+SHH(F)-E11.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E11.5-coronal , LHX1(D)+SHH(F)-E11.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E12.5-coronal , LHX1(D)+SHH(F)-E12.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		LHX1(D)+SHH(F)-E12.5-coronal , LHX1(D)+SHH(F)-E12.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Very strong	Non-Uniform	LHX1(D)+SHH(F)-E12.5-coronal , LHX1(D)+SHH(F)-E12.5-sagittal	There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Ambiguous		LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	There is strong staining in the mantle zone of the AVH and moderate staining in the anterior ID. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei and posterior ID.
TS20: hypothalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Strong	Non-Uniform	LHX1(D)+SHH(F)-E12.5-coronal , LHX1(D)+SHH(F)-E12.5-sagittal	There is strong staining in the mantle zone of the prethalamus and moderate staining in the dorsal thalamus.
TS20: thalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E12.5-sagittal , LHX1(D)+SHH(F)-E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		LHX1(D)+SHH(F)-E14.5-sagittal , LHX1(D)+SHH(F)-E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E14.5-coronal , LHX1(D)+SHH(F)-E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		LHX1(D)+SHH(F)-E14.5-sagittal , LHX1(D)+SHH(F)-E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Very strong	Non-Uniform	LHX1(D)+SHH(F)-E14.5-sagittal , LHX1(D)+SHH(F)-E14.5-coronal	There is very strong staining in the mantle zone of the mam zone. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E14.5-coronal , LHX1(D)+SHH(F)-E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Ambiguous		LHX1(D)+SHH(F)-E14.5-coronal , LHX1(D)+SHH(F)-E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Strong	Non-Uniform	LHX1(D)+SHH(F)-E14.5-coronal , LHX1(D)+SHH(F)-E14.5-sagittal	There is strong staining in the mantle zone of the AVH and anterior ID and moderate staining in the dorsal POA. Staining is either absent or ubiquitous in the mantle zone of the ventral POA, the PVH, VMH and ARH nuclei and posterior ID.
TS22: hypothalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E14.5-coronal , LHX1(D)+SHH(F)-E14.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Strong		LHX1(D)+SHH(F)-E14.5-sagittal , LHX1(D)+SHH(F)-E14.5-coronal	There is strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E14.5-sagittal , LHX1(D)+SHH(F)-E14.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		LHX1(D)+SHH(F)-E16.5-sagittal , LHX1(D)+SHH(F)-E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		LHX1(D)+SHH(F)-E16.5-sagittal , LHX1(D)+SHH(F)-E16.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Very strong	Non-Uniform	LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	There is very strong staining in the ventricular zone of the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones.
TS24: ganglionic eminence	Ambiguous		LHX1(D)+SHH(F)-E16.5-sagittal , LHX1(D)+SHH(F)-E16.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	There is very strong staining in the MZ of the SCH nucleus and moderate staining in the DAH and DMH nucleus. Staining is either absent or ubiquitous in the MZ of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and the PH.
TS24: hypothalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Strong		LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	There is strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		LHX1(D)+SHH(F)-E16.5-coronal , LHX1(D)+SHH(F)-E16.5-sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.