Gene Expression Data
Assay Details
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Assay
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Reference:
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J:157819
Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4452072
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Gene symbol:
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Arx
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Gene name:
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aristaless related homeobox
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Probe:
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UI-M-BH3-awh-f-01-0-UI.s1
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
3b:
embryonic day 12.5
(more  )
Note: Double labeled: brown - Arx; blue - Shh (assay MGI:4452070).
Specimen
3b:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Arx; blue - Shh (assay MGI:4452070).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
3b
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Expression is present in the prethalamus.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3b
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Expression is present in two distinctive regions in hypothalamic neuroepithelium; one extends towards the ventral hypothalamic surface and the other runs towards the optic recess.
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Specimen
3c:
embryonic day 12.5
(more )
Note: Double labeled: brown - Arx; blue - Nkx2-1 (assay MGI:4450737).
Specimen
3c:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Arx; blue - Nkx2-1 (assay MGI:4450737).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
3d
,
3c
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Expression is present in the prethalamus.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3c
,
3d
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Expression is present in two distinctive regions in the hypothalamic neuroepithelium.
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Specimen
3e:
embryonic day 12.5
(more )
Note: Double labeled: brown - Arx; blue - Lhx6 (assay MGI:4450765).
Specimen
3e:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: brown - Arx; blue - Lhx6 (assay MGI:4450765).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3e
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Expression is present in the tubermammillary terminal.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3e
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Expression is present in the intrahypothalamic diagonal.
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Specimen
3i:
embryonic day 12.5
(more )
Note: Triple labeled: brown - Arx; blue - Olig2 (assay MGI:4452076 and Lhx9 (assay MGI:4450767).
Specimen
3i:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: brown - Arx; blue - Olig2 (assay MGI:4452076 and Lhx9 (assay MGI:4450767).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
3i
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Expression is present in the prethalamus.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3i
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Expression is present in two distinctive regions in the hypothalamic neuroepithelium.
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Specimen
3l:
embryonic day 12.5
(more )
Note: Double labeled: brown - Arx; blue - Sim1 (assay MGI:4450888).
Specimen
3l:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
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Note:
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Double labeled: brown - Arx; blue - Sim1 (assay MGI:4450888).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
3l inset
,
3l
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Expression is present in the prethalamus.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3l
,
3l inset
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Expression is present in two distinctive regions in the hypothalamic neuroepithelium.
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Specimen
3m:
embryonic day 12.5
(more )
Note: Double labeled: brown - Arx; blue - Nkx6-2 (assay MGI:4450786).
Specimen
3m:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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|
Note:
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Double labeled: brown - Arx; blue - Nkx6-2 (assay MGI:4450786).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thalamus |
Present |
Regionally restricted |
3m
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Expression is present in the prethalamus.
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| TS20: hypothalamus |
Present |
Regionally restricted |
3m
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Expression is present in two distinctive regions in the hypothalamic neuroepithelium.
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Analysis Tools