Gene Expression Data
Assay Details
Assay
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Reference:
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J:157819
Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4452075
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Gene symbol:
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Lhx6
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Gene name:
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LIM homeobox protein 6
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Probe:
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MGC clone 85996
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Alkaline phosphatase
|
|
Results
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Specimen
3j:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Nkx2-1 (assay MGI:4450737).
Specimen
3j:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Nkx2-1 (assay MGI:4450737).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
3j
|
Expression is present in the intrahypothalamic diagonal.
|
TS20: diencephalon |
Present |
Regionally restricted |
3j
|
Expression is present in the tubermammillary terminal.
|
Specimen
4a:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Foxb1 (assay MGI:4450814).
Specimen
4a:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Foxb1 (assay MGI:4450814).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
4a
|
Expression is present in the tuberomammillary terminal.
|
Specimen
4b:
embryonic day 16.5
(more )
Note: Double labeled: brown - Lhx6; blue - Foxb1 (assay MGI:4450814).
Specimen
4b:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Foxb1 (assay MGI:4450814).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
4b
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4c:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Irx5 (assay MGI:4450789).
Specimen
4c:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Irx5 (assay MGI:4450789).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
4c
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4d:
embryonic day 16.5
(more )
Note: Double labeled: brown - Lhx6; blue - Irx5 (assay MGI:4450789).
Specimen
4d:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Irx5 (assay MGI:4450789).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
4d
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4e:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Lef1 (assay MGI:4450873).
Specimen
4e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Lef1 (assay MGI:4450873).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
4e
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4f:
embryonic day 16.5
(more )
Note: Double labeled: brown - Lhx6; blue - Lef1 (assay MGI:4450873).
Specimen
4f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Lef1 (assay MGI:4450873).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
4f
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4g:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Nr5a1 (assay MGI:4452112).
Specimen
4g:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Nr5a1 (assay MGI:4452112).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
4g
|
Expression is present in the tuberomammilary terminal.
|
Specimen
4h:
embryonic day 16.5
(more )
Note: Double labeled: brown - Lhx6; blue - Nr5a1 (assay MGI:4452112).
Specimen
4h:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Nr5a1 (assay MGI:4452112).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: diencephalon |
Present |
Regionally restricted |
4h
|
Expression is present in the tuberomammilary terminal.
|
Specimen
5c:
postnatal day 6
(more )
Note: Double labeled: brown - Lhx6; blue - Lhx9 (assay MGI:4450767).
Specimen
5c:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Lhx9 (assay MGI:4450767).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hypothalamus |
Present |
Regionally restricted |
5c
|
Expression is present in the lateral and dorsomedial hypothalamus, including in the intrahypothalamic diagonal.
|
Specimen
5d:
embryonic day 12.5
(more )
Note: Double labeled: brown - Lhx6; blue - Lhx8 (assay MGI:4450748).
Specimen
5d:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Lhx8 (assay MGI:4450748).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
Regionally restricted |
5d
|
Expression is present in the posterior portion of the intrahypothalamic diagonal.
|
Specimen
5e:
embryonic day 16.5
(more )
Note: Double labeled: brown - Lhx6; blue - Lhx8 (assay MGI:4450748).
Specimen
5e:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Lhx8 (assay MGI:4450748).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hypothalamus |
Present |
Regionally restricted |
5e
|
Expression is present in the intrahypothalamic diagonal.
|
Specimen
5f:
postnatal day 6
(more )
Note: Double labeled: brown - Lhx6; blue - Gal (assay MGI:4452125).
Specimen
5f:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Gal (assay MGI:4452125).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hypothalamus |
Present |
Regionally restricted |
5f
|
Expression is present in the intrahypothalamic diagonal.
|
Specimen
5i:
postnatal day 6
(more )
Note: Double labeled: brown - Lhx6; blue - Hcrt (assay MGI:4452128).
Specimen
5i:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: brown - Lhx6; blue - Hcrt (assay MGI:4452128).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hypothalamus |
Present |
Regionally restricted |
5i
|
Expression is present in the intrahypothalamic diagonal.
|
|