Probe:	Sulf2_#15
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		1Q
TS20: trunk mesenchyme	Present	Regionally restricted	1Q	Expression was detected in the mesenchyme surrounding the cartilage primordia of the vertebrae.

Structure	Level	Pattern	Image	Note
TS22: basioccipital cartilage condensation	Present	Regionally restricted	1R	Expression was detected in chondrocytes of the parachrodal plate (future basioccipital and basisphenoid bones).
TS22: basisphenoid cartilage condensation	Present	Regionally restricted	1R	Expression was detected in chondrocytes of the parachrodal plate (future basioccipital and basisphenoid bones).
TS22: vertebral cartilage condensation	Present	Regionally restricted	1R	Expression was detected in chondrocytes of the vertebrae.
TS22: brainstem	Present		1R

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		1W
TS20: trunk mesenchyme	Present	Regionally restricted	1W	Expression was detected in the mesenchyme surrounding the cartilage primordia of the vertebrae.

Structure	Level	Pattern	Image	Note
TS22: basioccipital cartilage condensation	Present	Regionally restricted	1X	Expression was detected in chondrocytes of the parachrodal plate (future basioccipital and basisphenoid bones).
TS22: basisphenoid cartilage condensation	Present	Regionally restricted	1X	Expression was detected in chondrocytes of the parachrodal plate (future basioccipital and basisphenoid bones).
TS22: vertebral cartilage condensation	Present	Regionally restricted	1X	Expression was detected in chondrocytes of the vertebrae.

Structure	Level	Pattern	Image	Note
TS20: lung mesenchyme	Present	Regionally restricted	2C	Expression was detected in the lung mesenchyme, close to the bronchial airway epithelium.
TS20: gut mesentery	Present		2C
TS20: stomach mesentery	Present		2C
TS20: ventricle cardiac muscle	Present		2C

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present	Regionally restricted	2D	Expression was detected in the lung mesenchyme, close to the bronchial airway epithelium.
TS22: esophagus	Present	Regionally restricted	2D	Expression was detected in the innervating neural progenitors of the esophagus.
TS22: ventricle cardiac muscle	Present		2D

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present	Regionally restricted	2E	Expression was detected in the lung mesenchyme, close to the bronchial airway epithelium.

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present	Regionally restricted	2I	Expression was detected in the nephrogenic mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: esophagus	Present		2J
TS22: stomach	Present		2J
TS22: spleen primordium	Present	Regionally restricted	2J	Expression was detected in the splenic mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3F	Expression was detected in the cortical hem.
TS22: choroid plexus	Absent		3F
TS22: Meckel's cartilage	Present		3F
TS22: otic capsule	Present		3F
TS22: head mesenchyme	Present	Widespread	3F	Expression was widespread in the head mesenchyme and surrounding the parachordal plate (future basioccipital and basisphenoid bones).

Structure	Level	Pattern	Image	Note
TS22: spinal cord floor plate	Present		3J

Structure	Level	Pattern	Image	Note
TS22: perioptic mesenchyme	Present		4F

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	4L	Expression was detected in the outer neuroblastic layer.
TS24: retina	Present	Regionally restricted	4L	Expression was intense in the optic cup margin.
TS24: perioptic mesenchyme	Present		4L

Structure	Level	Pattern	Image	Note
TS22: ulna cartilage condensation	Present	Regionally restricted	5F	Expression was detected in proliferating chondrocytes.
TS22: elbow joint primordium	Present	Regionally restricted	5F	Expression was detected in articular chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: ulna	Present	Regionally restricted	5L	Expression was detected in proliferating chondrocytes and in the developing bone.
TS24: elbow joint	Present		5L

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present		6C

Structure	Level	Pattern	Image	Note
TS21: limb digit	Present		6D

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	6I	Expression was detected in the tip of the digits.

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Present	Regionally restricted	6J	Expression was detected in proliferating chondrocytes.