Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149642
Hao L, et al., The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome. Biol Reprod. 2009 May;80(5):944-53
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4947648
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Gene symbol:
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Hspa5
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Gene name:
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heat shock protein 5
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Antibody:
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Anti-KDEL
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
5 KK morula:
embryonic day 3.0
(more  )
Note: Double labeled: green - Hspa5; red - Herpud1 (assay MGI:4947645).
Specimen
5 KK morula:
(close  )
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Genetic Background:
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DDK
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hspa5; red - Herpud1 (assay MGI:4947645).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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5_KK morula Anti-KDEL
,
5_KK morula Merge
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This protein is an integral part of the endoplasmic reticulum.
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Specimen
5 KB morula:
embryonic day 3.0
(more )
Note: Double labeled: green - Hspa5; red - Herpud1 (assay MGI:4947645).
Specimen
5 KB morula:
(close )
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Genetic Background:
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(DDK x C57BL/6J)F1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hspa5; red - Herpud1 (assay MGI:4947645).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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5_KB morula Anti-KDEL
,
5_KB morula merge
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This protein is an integral part of the endoplasmic reticulum. There are areas in the KB morulas in which expression appears to be more prominent than in the KK embryos.
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Specimen
6 KK morula:
embryonic day 3.0
(more )
Note: Double labeled: green - Hspa5; red - Atf4 (assay MGI:4947647).
Specimen
6 KK morula:
(close )
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Genetic Background:
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DDK
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hspa5; red - Atf4 (assay MGI:4947647).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
|
6_KK morula Anti-KDEL
,
6_KK morula Merge
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This protein is an integral part of the endoplasmic reticulum.
|
Specimen
6 KB morula:
embryonic day 3.0
(more )
Note: Double labeled: green - Hspa5; red - Atf4 (assay MGI:4947647).
Specimen
6 KB morula:
(close )
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Genetic Background:
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(DDK x C57BL/6J)F1
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Hspa5; red - Atf4 (assay MGI:4947647).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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6_KB morula Anti-KDEL
,
6_KB morula Merge
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This protein is an integral part of the endoplasmic reticulum. There are areas in the KB morulas in which expression appears to be more prominent than in the KK embryos.
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Analysis Tools