Gene Expression Data
Assay Details
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Assay
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Reference:
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J:171106
Suzuki-Hirano A, et al., Dynamic spatiotemporal gene expression in embryonic mouse thalamus. J Comp Neurol. 2011 Feb 15;519(3):528-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5294801
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Gene symbol:
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Gria1
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Gene name:
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glutamate receptor, ionotropic, AMPA1 (alpha 1)
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Probe:
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iGluR1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
6A:
embryonic day 12.5
(more  )
Note: Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
Specimen
6A:
(close  )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon mantle layer |
Strong |
Regionally restricted |
6A
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Expression is present throughout the most superficial region of the prosomere 2 region, the anterior half of the mantle region.
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| TS20: diencephalon |
Present |
Regionally restricted |
6A
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Expression is present in a subdomain of prosomere 3.
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Specimen
6B:
embryonic day 12.5
(more )
Note: Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
Specimen
6B:
(close )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon mantle layer |
Strong |
Regionally restricted |
6B
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Expression is present throughout the most superficial region of the prosomere 2 region, the anterior half of the mantle region.
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| TS20: diencephalon |
Present |
Regionally restricted |
6B
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Expression is present in a subdomain of prosomere 3.
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Specimen
6C:
embryonic day 12.5
(more )
Note: Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
Specimen
6C:
(close )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon mantle layer |
Strong |
Regionally restricted |
6C
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Expression is present throughout the most superficial region of the prosomere 2 region, the anterior half of the mantle region.
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| TS20: diencephalon |
Present |
Regionally restricted |
6C
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Expression is present in a subdomain of prosomere 3.
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Specimen
6D:
embryonic day 12.5
(more )
Note: Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
Specimen
6D:
(close )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Embedding:
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Cryosection
|
|
Note:
|
Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon mantle layer |
Strong |
Regionally restricted |
6D
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Expression is present throughout the most superficial region of the prosomere 2 region, the anterior half of the mantle region.
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| TS20: diencephalon |
Present |
Regionally restricted |
6D
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Expression is present in a subdomain of prosomere 3.
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Specimen
6E:
embryonic day 12.5
(more )
Note: Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
Specimen
6E:
(close )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: blue - Gria1; brown - Shh (assay MGI:5294438).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon mantle layer |
Strong |
Regionally restricted |
6E
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Expression is present throughout the most superficial region of the prosomere 2 region, the anterior half of the mantle region.
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| TS20: diencephalon |
Present |
Regionally restricted |
6E
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Expression is present in a subdomain of prosomere 3.
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Specimen
Not shown:
embryonic day 15.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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Slc:ICR
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: prethalamus |
Present |
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Expression remains only in the prethalamus; it has been lost in prosomere 2.
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Analysis Tools