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Gene Expression Data
Assay Details
Assay
Reference: J:143379 Hirasawa R, et al., Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos. Gene Expr Patterns. 2009 Jan;9(1):27-30
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5431442
Gene symbol: Dnmt3b
Gene name: DNA methyltransferase 3B
Antibody: anti-Dnmt3b (IMG-184)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A E3.0: embryonic day 3.0 (more )
Note: Double labeled: green - Dnmt3b; red - Pou5f1 (assay MGI:5431443).
Structure Level Pattern Image Note
TS3: compacted morula Present 1A E3.0 Dnmt3b , 1A E3.0 Dnmt3b/Oct4
Specimen 1A E3.5: embryonic day 3.5 (more )
Note: Double labeled: green - Dnmt3b; red - Pou5f1 (assay MGI:5431443).
Structure Level Pattern Image Note
TS4: trophectoderm Present 1A E3.5 Dnmt3b/Oct4 , 1A E3.5 Dnmt3b
TS4: inner cell mass Absent 1A E3.5 Dnmt3b , 1A E3.5 Dnmt3b/Oct4
Specimen 1A E4.0: embryonic day 4.0 (more )
Note: Double labeled: green - Dnmt3b; red - Pou5f1 (assay MGI:5431443).
Structure Level Pattern Image Note
TS5: polar trophectoderm Present 1A E4.0 Dnmt3b , 1A E4.0 Dnmt3b/Oct4
TS5: mural trophectoderm Absent 1A E4.0 Dnmt3b , 1A E4.0 Dnmt3b/Oct4
Specimen 2A top: embryonic day 4.5 (more )
Note: Double labeled: green - Dnmt3b; red - Pou5f1 (assay MGI:5431443).
Structure Level Pattern Image Note
TS6: polar trophectoderm Strong 2A E4.5 top Dnmt3b/Oct4 , 2A E4.5 top Dnmt3b
TS6: primitive endoderm Present 2A E4.5 top Dnmt3b/Oct4 , 2A E4.5 top Dnmt3b
TS6: embryo Present Single cells 2A E4.5 top Dnmt3b/Oct4 , 2A E4.5 top Dnmt3b
Specimen 2A bottom: embryonic day 4.5 (more )
Note: Double labeled: green - Dnmt3b; red - Gata4 (assay MGI:5431444).
Structure Level Pattern Image Note
TS6: polar trophectoderm Present 2A E4.5 bottom Dnmt3b/Gata4 , 2A E4.5 bottom Dnmt3b
TS6: primitive endoderm Present 2A E4.5 bottom Dnmt3b/Gata4 , 2A E4.5 bottom Dnmt3b
TS6: embryo Present Single cells 2A E4.5 bottom Dnmt3b/Gata4 , 2A E4.5 bottom Dnmt3b
Specimen 2A: embryonic day 5.5 (more )
Note: Double labeled: green - Dnmt3b; red - Gata4 (assay MGI:5431444).
Structure Level Pattern Image Note
TS8: epiblast Present 2A E5.5 Dnmt3b , 2A E5.5 Dnmt3b/Gata4
TS8: extraembryonic component Absent 2A E5.5 Dnmt3b/Gata4 , 2A E5.5 Dnmt3b
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
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