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Gene Expression Data
Assay Details
Assay
Reference: J:143379 Hirasawa R, et al., Dynamic transition of Dnmt3b expression in mouse pre- and early post-implantation embryos. Gene Expr Patterns. 2009 Jan;9(1):27-30
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5431443
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct-3/4 (H-134; sc-9081)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1A E3.0: embryonic day 3.0 (more )
Note: Double labeled: red - Pou5f1; green- Dnmt3b (assay MGI:5431442).
Structure Level Pattern Image Note
TS3: compacted morula Present 1A E3.0 Dnmt3b/Oct4 , 1A E3.0 Oct4

Specimen 1A E3.5: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green- Dnmt3b (assay MGI:5431442).
Structure Level Pattern Image Note
TS4: inner cell mass Present 1A E3.5 Dnmt3b/Oct4 , 1A E3.5 Oct4

Specimen 1A E4.0: embryonic day 4.0 (more )
Note: Double labeled: red - Pou5f1; green- Dnmt3b (assay MGI:5431442).
Structure Level Pattern Image Note
TS5: inner cell mass Present 1A E4.0 Oct4 , 1A E4.0 Dnmt3b/Oct4

Specimen 2A top: embryonic day 4.5 (more )
Note: Double labeled: red - Pou5f1; green- Dnmt3b (assay MGI:5431442).
Structure Level Pattern Image Note
TS6: blastocyst Present 2A E4.5 top Dnmt3b/Oct4 , 2A E4.5 top Oct4


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
12/17/2024
MGI 6.24
The Jackson Laboratory