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Gene Expression Data
Assay Details
Assay
Reference: J:42028 Seitanidou T, et al., Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech Dev. 1997 Jul;65(1-2):31-42
Assay type: RNA in situ
MGI Accession ID: MGI:5436406
Gene symbol: Hoxb1
Gene name: homeobox B1
Probe: Hox-2.9 probe3
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3C: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 8 somites. Also stained for Hoxb3 (assay MGI:5436405).
Structure Level Pattern Image Note
TS13: rhombomere 4 Present 3C
Specimen 3F: embryonic day 8.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: 10-12 somites. Also stained for Hoxb3 (assay MGI:5436405).
Structure Level Pattern Image Note
TS13: rhombomere 4 Present 3F
Specimen 4C: embryonic day 10.5; Egr2tm1Pch/Egr2+ (more )
Note: Double labeled: purple - Hoxb1; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Structure Level Pattern Image Note
TS17: rhombomere 4 Present 4C
Specimen 4D: embryonic day 10.5; Egr2tm1Pch/Egr2tm1Pch (more )
Note: Double labeled: purple - Hoxb1; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Structure Level Pattern Image Note
TS17: rhombomere 4 Present 4D The domain of expression was reduced. In the ventral region the width of the domain was about 80% that of the control. The effect was even more pronounced in the dorsal part.
Specimen not shown: embryonic day 9.5; Egr2tm1Pch/Egr2tm1Pch (more )
Structure Level Pattern Image Note
TS15: rhombomere 4 Present Regionally restricted The domain of expression was reduced.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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