Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:42028
Seitanidou T, et al., Krox-20 is a key regulator of rhombomere-specific gene expression in the developing hindbrain. Mech Dev. 1997 Jul;65(1-2):31-42
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5436418
|
Gene symbol:
|
Fst
|
Gene name:
|
follistatin
|
Probe:
|
follistatin probe1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
4E:
embryonic day 8.0; Egr2tm1Pch/Egr2 +
(more )
Note: 7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
4E
|
Expression was detected in rhombomere 2 and 4.
|
Specimen
4F:
embryonic day 8.0; Egr2tm1Pch/ Egr2tm1Pch
(more )
Note: 7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
Specimen
4F:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
7-8 somites. Double labeled: purple - Fst; turquoise/blue - Egr2 (Krox20; assay MGI:5436312).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
4F
|
Expression was detected in a continuous rhombomere 2,3,4 domain extending caudally to the rhombomere 4/5 boundary. The staining hid Egr2 lacZ staining in rhombomere 3 (see assay MGI:5436312).
|
Specimen
4G:
embryonic day 8.0
(more )
Note: 6 somites.
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
6 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
4G
|
Expression was detected in rhombomere 2 and 4.
|
Specimen
4H:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
6 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: rhombomere |
Present |
Regionally restricted |
4H
|
Expression was detected in all cells within the rhombomere 2,3,4 domain, but not in the prospective rhombomere 5 territory.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6 * DBA/2
|
Mutant Allele(s):
|
Egr2tm1Pch/Egr2tm1Pch
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
12 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: rhombomere 2 |
Present |
|
|
|
TS13: rhombomere 4 |
Present |
|
|
|
TS13: rhombomere 5 |
Absent |
|
|
|
TS13: rhombomere |
Present |
Regionally restricted |
|
Expression was maintained in rhombomere 2 and 4. At 12 somites, rhombomere 3 has almost disappeared in the mutant embryos. Rhombomere 5 remained negative, and a low signal appeared in rhombomere 6.
|
|