Antibody:	Anti-Mdm20
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS19: brain	Strong		1C

Structure	Level	Pattern	Image	Note
TS22: brain	Strong		1D
TS22: spinal cord	Strong		1D

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2A Mdm20/Nat5 low mag , 2A Mdm20/Nat5 , 2A Mdm20	Expression was detected in the dorsal telencephalon, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2B Mdm20/Nat5 low mag , 2B Mdm20 , 2B Mdm20/Nat5	Expression was detected in the ganglionic eminence, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2C Mdm20/Nat5 , 2C Mdm20 , 2C Mdm20/Nat5 low mag	Expression was detected in the preoptic area, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	2D Mdm20 , 2D Mdm20/Nat5 low mag , 2D Mdm20/Nat5	Expression was detected in the dorsal diencephalon, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	2E Mdm20/Nat5 low mag , 2E Mdm20 , 2E Mdm20/Nat5	Expression was detected in the posterior mesencephalon, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate	Present	Regionally restricted	2F Mdm20 , 2F Mdm20/Nat5 , 2F Mdm20/Nat5 low mag	Expression was detected in the pons, including in most PCNA-positive proliferating neural progenitors.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	4A Mdm20 , 4A Mdm20/TuJ1	Expression was detected in proliferating progenitors in the ventricular zone and in early neurons in the mantle layer of the ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	4B Mdm20/TuJ1 , 4B Mdm20	Expression was detected in proliferating progenitors in the ventricular zone and in early neurons in the mantle layer of the ganglionic eminence

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata ventricular layer	Present		4C Mdm20 , 4C Mdm20/TuJ1
TS20: medulla oblongata mantle layer	Present		4C Mdm20 , 4C Mdm20/TuJ1

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Present	Non-Uniform	5A Mdm20 Nat5 DAPI , 5A Mdm20 , 5A Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Non-Uniform	5B Mdm20 , 5B Mdm20 Nat5 Map2 , 5B Mdm20 Map2 , 5B Mdm20 Nat5 , 5B Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: hippocampus	Present	Non-Uniform	5C Mdm20 Nat5 , 5C Mdm20 , 5C Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: corpus striatum	Present	Non-Uniform	5D Mdm20 , 5D Mdm20 Nat5 DAPI , 5D Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: ganglionic eminence	Present	Non-Uniform	5E Mdm20 , 5E Mdm20 Nat5 , 5E Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: caudal ganglionic eminence	Present	Regionally restricted	5F Mdm20 , 5F Mdm20 Nat5 DAPI , 5F Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: preoptic area	Present	Regionally restricted	5G Mdm20 Nat5 DAPI , 5G Mdm20 Nat5 , 5G Mdm20	Expression was detected in differentiating neurons in the preoptic area. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	5H Mdm20 , 5H Mdm20 Map2 , 5H Mdm20 Nat5 DAPI , 5H Mdm20 Nat5 , 5H Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the bed nucleus stria terminalis and zona incerta. Co-expression with Nat5 (Naa20) and Map2 was noted.
TS22: zona incerta	Present	Regionally restricted	5H Mdm20 , 5H Mdm20 Map2 , 5H Mdm20 Nat5 DAPI , 5H Mdm20 Nat5 , 5H Mdm20 Nat5 Map2	Expression was detected in differentiating neurons of the bed nucleus stria terminalis and zona incerta. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present	Non-Uniform	5I Mdm20 , 5I Mdm20 Nat5 DAPI , 5I Mdm20 Nat5	Expression was detected in differentiating neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present	Regionally restricted	6A Mdm20 , 6A Mdm20 Nat5 DAPI , 6A Mdm20 Nat5	Expression was detected in differentiating neurons of the dorsal raphe of the mesencephalon. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6B Mdm20 , 6B Mdm20 Nat5 DAPI , 6B Mdm20 Nat5	Expression was detected in differentiating neurons of the principal sensory trigeminal nucleus. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6C Mdm20 Nat5 , 6C Mdm20 Nat5 DAPI , 6C Mdm20	Expression was detected in differentiating neurons of the facial and vestibular nuclei. Co-expression with Nat5 (Naa20) was noted in the vestibular nucleus.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	6D Mdm20 , 6D Mdm20 Nat5 Map2 , 6D Mdm20 Nat5 DAPI , 6D Mdm20 Nat5 , 6D Mdm20 Map2	Expression was detected in differentiating neurons of the cochlear nucleus. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Present	Regionally restricted	6E Mdm20 Nat5 DAPI , 6E Mdm20 Nat5 , 6E Mdm20	Expression was detected in differentiating neurons of the cerebellar deep neurons.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata basal plate	Present	Regionally restricted	6F Mdm20 , 6F Mdm20 Nat5 Map2 , 6F Mdm20 Nat5 , 6F Mdm20 Nat5 DAPI , 6F Mdm20 Map2	Expression was detected in differentiating neurons of the medullary raphe. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6G Mdm20 , 6G Mdm20 Nat5 , 6G Mdm20 Nat5 DAPI	Expression was detected in differentiating neurons of the spinal trigeminal nucleus. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	6H Mdm20 Map2 , 6H Mdm20 Nat5 Map2 , 6H Mdm20 Nat5 DAPI , 6H Mdm20 Nat5 , 6H Mdm20	Expression was detected in differentiating neurons of the external cuneate nucleus. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Non-Uniform	7F , 7A	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.
TS26: lateral ventricle ependyma	Present	Non-Uniform	7B , 7A	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Present	Non-Uniform	7C , 7H	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: accumbens nucleus	Present	Non-Uniform	7I , 7D	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: zona incerta	Present	Regionally restricted	7E , 7J	Expression was detected in differentiated neurons in the zona incerta. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: pineal gland	Present	Non-Uniform	7G	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: cochlear nucleus	Present	Regionally restricted	7K , 7O	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7P , 7L	Expression was detected in differentiated neurons of the pontine reticular formation. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	7Q , 7M	Expression was detected in differentiated neurons of the external cuneate nucleus. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: inferior olivary nucleus	Present	Regionally restricted	7R , 7N	Expression was detected in differentiated neurons in the ventral portion of the inferior olivary nucleus. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Present	Non-Uniform	7S Mdm20 , 7S Merge	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: piriform cortex	Present	Non-Uniform	7T Mdm20 , 7T Merge	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: accumbens nucleus	Present	Non-Uniform	7U Mdm20 , 7U Merge	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: cochlear nucleus	Present	Regionally restricted	7V Mdm20 , 7V Merge	Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	7W Merge , 7W Mdm20	Expression was detected in differentiated neurons of the pontine reticular formation. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	7X Merge , 7X Mdm20	Expression was detected in differentiated neurons of the external cuneate nucleus. Co-expression with Nat5 (Naa20) and Map2 was noted.

Structure	Level	Pattern	Image	Note
TS26: amygdala	Present	Non-Uniform		Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.
TS26: medullary raphe	Present	Non-Uniform		Expression was detected in differentiated neurons. Co-expression with Nat5 (Naa20) was noted.
TS26: cerebral cortex	Present	Regionally restricted		Expression was detected in differentiated neurons of the cingulate cortex. Co-expression with Nat5 (Naa20) was noted.
TS26: midbrain	Present	Regionally restricted		Expression was detected in differentiated neurons in the interpeduncular nucleus and dorsal raphe. Co-expression with Nat5 (Naa20) was noted.
TS26: medulla oblongata	Present	Regionally restricted		Expression was detected in differentiated neurons in the spinal trigeminal nucleus. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: preoptic area	Present	Regionally restricted	8A	Expression was detected in preoptic area neurons. There was no co-expression with Nat5 (Naa20).

Structure	Level	Pattern	Image	Note
TS26: pons	Present	Regionally restricted	8B	Expression was detected in vestibular nucleus neurons. There was no co-expression with Nat5 (Naa20).

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	8C	Expression was detected in thalamic paraventricular nucleus neurons. There was no co-expression with Nat5 (Naa20).

Structure	Level	Pattern	Image	Note
TS26: medulla oblongata	Present	Regionally restricted	8D	Expression was detected in lateral reticular nucleus neurons. There was no co-expression with Nat5 (Naa20).

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	9A	Expression was detected in dopaminergic neurons in the ventral tegmental area. Co-expression with Nat5 (Naa20) was noted.
TS26: substantia nigra	Present	Regionally restricted	9A	Expression was detected in dopaminergic neurons in the substantia nigra. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	9B	Expression was detected in dopaminergic neurons in the ventral tegmental area. Co-expression with Nat5 (Naa20) was noted.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present	Regionally restricted	9C	Expression was detected in dopaminergic neurons in the ventral tegmental area. Co-expression with Tyrosine hydroxylase was noted.