Antibody:	anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	2_TuJ1 yellow bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate	Present	Regionally restricted	2_TuJ1 red bar	Expression was detected in early neurons in the posterior mesencephalon and pons.
TS19: midbrain	Present	Regionally restricted	2_TuJ1 red bar	Expression was detected in early neurons in the posterior mesencephalon and pons.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	4A TuJ1 , 4A Mdm20/TuJ1	Expression was detected in early differentiating neurons in the mantle layer.

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Present	Regionally restricted	4B TuJ1 , 4B Mdm20/TuJ1	Expression was detected in early differentiating neurons in the mantle layer.

Structure	Level	Pattern	Image	Note
TS20: medulla oblongata mantle layer	Present	Non-Uniform	4C Mdm20/TuJ1 , 4C TuJ1	Expression was detected in early differentiating neurons in the mantle layer.

Structure	Level	Pattern	Image	Note
TS22: telencephalon septum	Present	Regionally restricted	5_TuJ1 yellow bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	5_TuJ1 red bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	5_TuJ1 blue bar	Expression was detected in early neurons.
TS22: telencephalon	Present	Regionally restricted	5_TuJ1 blue bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS22: epithalamus	Present	Regionally restricted	5_TuJ1 green bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	6_TuJ1 blue bar	Expression was detected in early neurons in the upper brainstem.
TS22: midbrain	Present	Regionally restricted	6_TuJ1 blue bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	6_TuJ1 purple bar	Expression was detected in early neurons in the upper brainstem.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Present	Regionally restricted	6_TuJ1 orange bar	Expression was detected in early neurons in the lower brainstem.
TS22: cerebellum	Present	Regionally restricted	6_TuJ1 orange bar	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Present	Regionally restricted	7_TuJ1 yellow bar top	Expression was detected in early neurons.

Structure	Level	Pattern	Image	Note
TS26: pineal gland	Present	Regionally restricted	7_TuJ1 yellow bar bottom	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	7_TuJ1 blue bar	Expression was detected in neurons.

Structure	Level	Pattern	Image	Note
TS26: hindbrain	Present	Regionally restricted	7_TuJ1 green bar	Expression was detected in neurons.