Antibody:	Anti-Olf-1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Present	Regionally restricted	1	Expression in 2 clusters of basal cell nuclei.

Structure	Level	Pattern	Image	Note
TS20: telencephalon mantle layer	Present		2A
TS20: telencephalon ventricular layer	Absent		2A
TS20: hypothalamus	Present		2A	Discrete pattern of expression in the diencephalon in regions of future thalamus and hypothalamus.
TS20: thalamus	Present		2A	Discrete pattern of expression in the diencephalon in regions of future thalamus and hypothalamus.
TS20: medulla oblongata alar plate	Present		2A
TS20: metencephalon alar plate	Present		2A
TS20: metencephalon basal plate	Present		2A

Structure	Level	Pattern	Image	Note
TS20: alar columns	Present		2C	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: basal columns	Absent		2C	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: dorsal root ganglion	Present		2C
TS20: facial ganglion	Present		2C
TS20: glossopharyngeal nerve	Present		2C
TS20: trigeminal ganglion	Present		2C
TS20: vestibulo-cochlear ganglion complex	Present		2C

Structure	Level	Pattern	Image	Note
TS20: alar columns	Present		2D	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: basal columns	Absent		2D	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: dorsal root ganglion	Present	Regionally restricted	2D	Expression in sensory neurons within the dorsal root ganglia.
TS20: telencephalon	Present	Regionally restricted	2D	Expression at the margins of future olfactory bulbs.
TS20: olfactory epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS20: alar columns	Present		2E	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: basal columns	Absent		2E	Caudal to hindbrain, expression in dorsal (alar plate) but not ventral (basal plate) region of neural tube.
TS20: segmental spinal nerve	Present	Regionally restricted	2E	Expression in the four segmental nerves of the tail.
TS20: limb pre-cartilage condensation	Present		2E

Structure	Level	Pattern	Image	Note
TS20: telencephalon mantle layer	Present		2F
TS20: telencephalon ventricular layer	Absent		2F

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		2G

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	3A	Intense staining in a thin layer of cells within the cerebral cortex.
TS22: corpus striatum	Present	Diffuse	3A	Diffuse expression in primordium of the corpus striatum.
TS22: diencephalon	Present	Non-Uniform	3A
TS22: hindbrain	Strong		3A

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	3B	Intense staining in a thin layer of cells within the cerebral cortex.
TS22: main olfactory bulb	Present		3B
TS22: diencephalon	Present	Non-Uniform	3B
TS22: pons	Strong		3B

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Strong		3C	Intense staining in rhombencephalon, including the medulla oblongata.
TS22: neural retinal epithelium	Strong		3C	Highest levels of expression restricted to sensory structures, particularly olfactory epithelium and neural retina,
TS22: olfactory epithelium	Strong		3C	Highest levels of expression restricted to sensory structures, particularly olfactory epithelium and neural retina,

Structure	Level	Pattern	Image	Note
TS22: vomeronasal organ	Strong		3D	Highest levels of expression restricted to sensory structures, particularly vomeronasal organ.
TS22: inner ear	Present		3D
TS22: vibrissa	Present		3D
TS22: spinal cord	Present	Regionally restricted	3D	Expression in dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Regionally restricted	3E	Expression in dorsal spinal cord.

Structure	Level	Pattern	Image	Note
TS22: inner ear	Present		3F

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present	Regionally restricted	3G	Expression in the majority of neurons in the trigeminal ganglion, with most intense staining at the periphery.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong	Regionally restricted	4A	Robust staining in cells of the dorsal neural tube. Intense expression predominantly in sensory neurons of dorsal horns. Expression in only a few cells in intermediate zone and ventral horns.
TS20: spinal cord roof plate	Absent		4A

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Strong
TS20: spinal cord mantle layer	Present	Regionally restricted		Expression restricted to nuclei of postmitotic cells in mantle layer of neural tube.
TS20: spinal cord ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS27: spinal cord dorsal horn	Strong	Regionally restricted	4B	Intense expression predominantly in sensory neurons of dorsal horns, restricted to the outermost zones.

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Present	Non-Uniform	4C

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Non-Uniform	4D

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present	Non-Uniform	4E

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted	5A	Expression in neural retina, restricted to cells in central region of inner layer, adjacent to the optic nerve. No expression in outer layer.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	5B	Expression in a greater number of cells in inner layer, extending from central region towards peripheral margins. No expression in outer layer.
TS22: pigmented retinal epithelium	Absent		5B	No expression in pigmented retina or other parts of the eye.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Widespread	5C	Expression in most or all cells of inner layer.
TS24: retina nerve fiber layer	Absent		5C	No expression in outer layer.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		5D

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present		5E
TS27: retina inner nuclear layer	Present	Single cells	5E	Expression in a few cells in the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	5F	Expression in a discrete population of cells in the ganglion cell layer.
TS28: retina inner nuclear layer	Present	Single cells	5F	Expression in a few cells in inner half of the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: pigmented retinal epithelium	Absent			No expression in pigmented retina or other parts of the eye.

Structure	Level	Pattern	Image	Note
TS18: olfactory epithelium	Present		6A
TS18: nasal pit	Present	Regionally restricted	6A	Expression in anlage of vomeronasal gland in olfactory pit.,
TS18: nose	Present	Regionally restricted	6A	Expression in cells in proximity to olfactory axonal projections to the presumptive olfactory bulb.

Structure	Level	Pattern	Image	Note
TS18: dorsal root ganglion	Strong	Regionally restricted		Strong expression in a subset of presumptive sensory neurons in the dorsal root ganglion.
TS18: brain	Weak	Widespread		Faint expression throughout the brain.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Widespread	6B	Expression in olfactory turbinate throughout the epithelium.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	6C	Nuclear localization of expression.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	6D	Expression confined to lower 2/3rds of epithelium, corresponding to the neuronal cell layer.

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present	Regionally restricted	6E	Expression confined to lower 2/3rds of epithelium, corresponding to the neuronal cell layer.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	6F	Expression in cells close to the basement membrane.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present	Regionally restricted	6G	Expression in basal cells near the basal lamina.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	8J	Expression in nuclei.