Gene Expression Data
Assay Details
Assay
|
Reference:
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J:82630
Hall JM, et al., Disruption of sonic hedgehog signaling alters growth and patterning of lingual taste papillae. Dev Biol. 2003 Mar 15;255(2):263-77
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5504419
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Gene symbol:
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Bmp4
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Gene name:
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bone morphogenetic protein 4
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Reporter:
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lacZ
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Antibody:
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anti-B-galactosidase
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
1F/G:
embryonic day 13.5; Bmp4tm2Blh/Bmp4 +
(more )
Note: Double labeled: red - Bmp4 reporter; green - Shh (assay MGI:5504534).
Specimen
1F/G:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
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Mutant Allele(s):
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Bmp4tm2Blh/Bmp4+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Bmp4 reporter; green - Shh (assay MGI:5504534).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: tongue epithelium |
Present |
Regionally restricted |
1F/G
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Expressed in the developing papillary placodes.
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Specimen
2A/C:
embryonic day 12.0; Bmp4tm2Blh/Bmp4 +
(more )
Note: Double labeled: red - Bmp4 reporter; green - Uchl1 (PGP9.5; assay MGI:5504626).
Specimen
2A/C:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
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Mutant Allele(s):
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Bmp4tm2Blh/Bmp4+
|
Age:
|
embryonic day 12.0
|
Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Bmp4 reporter; green - Uchl1 (PGP9.5; assay MGI:5504626).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: tongue epithelium |
Present |
Regionally restricted |
2A
,
2C
|
Expressed in papillary placodal regions, which have not been innervated.
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Specimen
2D/F:
embryonic day 16.5; Bmp4tm2Blh/Bmp4 +
(more )
Note: Double labeled: red - Bmp4 reporter; green - Uchl1 (PGP9.5; assay MGI:5504626).
Specimen
2D/F:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
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Mutant Allele(s):
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Bmp4tm2Blh/Bmp4+
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Age:
|
embryonic day 16.5
|
Sex:
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Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Bmp4 reporter; green - Uchl1 (PGP9.5; assay MGI:5504626).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: fungiform papillae |
Present |
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2F
,
2D
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Expressed in fungiform papillary epithelium, which are extensively innervated.
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