Probe:	Alpha1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2A	Expression is on the ventricular side of the entire anterior neural tube.
TS19: cerebellum primordium	Present		2A

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Regionally restricted	2B	Expression is on the ventricular side of the entire anterior neural tube.
TS19: diencephalon	Present	Regionally restricted	2B	Expression is in the ventral hypothalamic primordium and the preoptic area.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2C	Expressed is stronger in the subventricular zone of the ventral pallial region than in the dorsal and medial pallium, where it was almost absent.

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	2D	Expressed is stronger in the subventricular zone of the ventral pallial region than in the dorsal and medial pallium, where it was almost absent.

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Present	Scattered	2N , 2M

Structure	Level	Pattern	Image	Note
TS22: midbrain ventricular layer	Strong		3A
TS22: midbrain lateral wall	Strong	Regionally restricted	3A	Expression is in the subventricular zone.
TS22: inferior colliculus	Strong	Regionally restricted	3A	Expression is in the mantle of the inferior colliculus.
TS22: cerebellum	Strong	Regionally restricted	3A	Expression is at the pial surface.
TS22: cerebellum ventricular layer	Present		3A
TS22: cerebral cortex subventricular zone	Present		3A
TS22: cerebral cortex ventricular layer	Present		3A
TS22: cortical plate	Present		3A
TS22: epithalamus	Present		3A
TS22: thalamus	Present	Regionally restricted	3A	Expression is in the caudal part.
TS22: cerebral cortex marginal layer	Absent		3A
TS22: pretectum	Absent		3A

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	3B	Expression is in the ventral areas (the subpallium, preoptic area).
TS22: epithalamus	Present		3B
TS22: ganglionic eminence	Present		3B
TS22: telencephalon septum	Present		3B
TS22: hypothalamus	Present	Regionally restricted	3B	Expression is in the infundibular and paraventricular region of the hypothalamus.
TS22: thalamus	Present	Regionally restricted	3B	Expression is in the caudal part.
TS22: pretectum	Absent		3B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present		3C
TS22: cerebral cortex ventricular layer	Present		3C
TS22: cortical plate	Present		3C
TS22: cerebral cortex marginal layer	Absent		3C

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present		3D

Structure	Level	Pattern	Image	Note
TS22: eye	Present		3M , 3N

Structure	Level	Pattern	Image	Note
TS25: hypothalamus	Present		4A
TS25: telencephalon	Present	Regionally restricted	4A	Expression is in the preoptic area.

Structure	Level	Pattern	Image	Note
TS25: thalamus	Strong		4B
TS25: midbrain lateral wall	Strong	Regionally restricted	4B	Expression is in the subventricular and ventricular zones.
TS25: cerebellum	Present		4B
TS25: hypothalamus	Present		4B
TS25: oculomotor nerve	Present		4B
TS25: telencephalon	Present	Regionally restricted	4B	Expression is in the preoptic area.
TS25: diencephalon	Weak	Regionally restricted	4B	Expression is in the prethalamus.

Structure	Level	Pattern	Image	Note
TS25: cortical plate	Strong		4C
TS25: cerebral cortex intermediate zone	Strong		4C	Expresssion is stronger in a deeper layer.
TS25: cerebral cortex marginal zone	Present		4C

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex subventricular zone	Strong		4D

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4M , 4N	Expression is in the more mature (apical) cell layers.

Structure	Level	Pattern	Image	Note
TS25: retina	Present		4Q , 4R	There is co-expression at the apical cell layer.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Strong		5A
TS28: cortical layer III	Strong		5A
TS28: cortical layer V	Strong	Regionally restricted	5A	Expression is in the upper part.
TS28: cortical layer VI	Strong	Regionally restricted	5A	Expression is in the middle part.
TS28: claustrum	Present		5A
TS28: corpus striatum	Present		5A
TS28: facial nucleus	Present		5A
TS28: inferior olivary nucleus	Present		5A
TS28: locus coeruleus	Present		5A
TS28: telencephalon septum	Present		5A

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present	Regionally restricted	5B	Expression is in the rostral migratory stream and in the different layers.
TS28: rostral migratory stream	Present	Regionally restricted	5B	Expression is in the rostral migratory stream and in the different layers.
TS28: main olfactory bulb glomerular layer	Strong		5B

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		5C	Strongest expression was found in the deeper parts.
TS28: cerebellum internal granule cell layer	Present	Scattered	5C
TS28: deep cerebellar nucleus	Present	Scattered	5C
TS28: cerebellum Purkinje cell layer	Absent		5C

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Strong		5D
TS28: cortical layer III	Strong		5D
TS28: cortical layer V	Strong	Regionally restricted	5D	Expression is in the upper part.
TS28: cortical layer VI	Strong	Regionally restricted	5D	Expression is in the middle part.

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present		5E

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Present		5F	Strongest expression was found in the deeper parts.
TS28: cerebellum internal granule cell layer	Present	Scattered	5F
TS28: cerebellum Purkinje cell layer	Absent		5F

Structure	Level	Pattern	Image	Note
TS28: forebrain	Present	Regionally restricted	5G	Expression is in the preoptic area.
TS28: epithalamus	Present		5G
TS28: habenula	Present		5G
TS28: hypothalamus	Present		5G
TS28: mammillary body	Present		5G
TS28: thalamus	Present		5G
TS28: midbrain	Present	Regionally restricted	5G	Expression is in the precommisural region of the pretectum.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Uniform	5H

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5I