Gene Expression Data
Assay Details
Assay
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Reference:
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J:93087
Bajanca F, et al., Integrins in the mouse myotome: developmental changes and differences between the epaxial and hypaxial lineage. Dev Dyn. 2004 Oct;231(2):402-15
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5528335
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Gene symbol:
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Itga6
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Gene name:
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integrin alpha 6
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Antibody:
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anti-alpha6 integrin (GoH3)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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The fixative was 0.2% paraformaldehyde.
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Results
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Specimen
2A:
embryonic day 11.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C:
embryonic day 11.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
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section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: somite |
Present |
Regionally restricted |
2C
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Expression was present on the surface of cells in epithelial somites.
|
Specimen
2D:
embryonic day 9.0
(more )
Note: 15 somite embryo. Double labeled: green - Itga6; red - Myf5 (assay MGI:5528358).
Specimen
2D:
(close )
Genetic Background:
|
CD-1
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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15 somite embryo. Double labeled: green - Itga6; red - Myf5 (assay MGI:5528358).
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: myotome |
Present |
|
2D
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Expression was stronger in the myotome than in the dermomyotome. All Myf-5 positive cells in the myotome were also alpha-6 positive.
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TS14: dermomyotome |
Present |
|
2D
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Expression was present in the whole dermomyotome. All Myf5-positive cells in the dorsomedial lip of the dermomyotome also express alpha-6.
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Specimen
2E:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Pax3 (assay MGI:5528359).
Specimen
2E:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Pax3 (assay MGI:5528359).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dermomyotome |
Strong |
|
2E
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Expression increased in the dermomyotome compared to the 15-somite stage.
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Specimen
2F:
embryonic day 9.5
(more )
Note: Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
Specimen
2F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: myotome |
Present |
|
2F
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Expression was detected on Myogenin-positive cells in the myotome.
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Specimen
2K:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
Specimen
2K:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
|
Specimen
2L:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
Specimen
2L:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: myotome |
Weak |
Regionally restricted |
2L
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Expression was weaker, almost absent, in rostral myotomes.
|
Specimen
3A:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: myotome |
Present |
|
3A
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There was no overlap with MyoD-expressing cells in young epaxial cells near the dorsomedial lip.
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Specimen
3B:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: myotome |
Present |
|
3B
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There was no overlap with MyoD-expressing cells in rostral mature myotomes. The hypaxial domain was negative for expression.
|
Specimen
3C:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
Specimen
3C:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myod1 (assay MGI:5528398).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: trunk myotome |
Present |
|
3C
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Some MyoD-positive nuclei are detected in trunk myotomes in areas containing alpha-6-positive elongated myocytes.
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Specimen
3D/F:
embryonic day 11.5
(more )
Note: Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
Specimen
3D/F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Itga6; red - Myog (assay MGI:5528360).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: myotome |
Present |
Regionally restricted |
3D
,
3F
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At the interlimb level of the trunk, there was strong expression in the intercalated myotome but faint expression in the ventrolateral (hypaxial) portion of the myotome compared to Myogenin staining the whole myotome.
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