Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:137168 Gordon EJ, et al., Expression of the hyaluronan receptor LYVE-1 is not restricted to the lymphatic vasculature; LYVE-1 is also expressed on embryonic blood vessels. Dev Dyn. 2008 Jul;237(7):1901-9
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5555942
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-Actin
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

2_E9.5 LYVE-1/SMA ©

Specimen 2 E9.5 LYVE-1: embryonic day 9.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present	Regionally restricted	2_E9.5 LYVE-1/SMA	Expression is in smooth muscle in the blood vessels.

2_E11.5 LYVE-1/SMA ©

Specimen 2 E11.5 LYVE-1: embryonic day 11.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS19: yolk sac	Present	Regionally restricted	2_E11.5 LYVE-1/SMA	Expression is in smooth muscle in the blood vessels.

2_E14.5 LYVE-1/SMA ©

Specimen 2 E14.5 LYVE-1: embryonic day 14.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS22: yolk sac	Present	Regionally restricted	2_E14.5 LYVE-1/SMA	Expression is in smooth muscle in the blood vessels.

2_E18.5 LYVE-1/SMA ©

Specimen 2 E18.5 LYVE-1: embryonic day 18.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS26: yolk sac	Present	Regionally restricted	2_E18.5 LYVE-1/SMA	Expression is in smooth muscle in the blood vessels.

2_E9.5 CD31/SMA ©

Specimen 2 E9.5 CD31: embryonic day 9.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present	Regionally restricted	2_E9.5 CD31/SMA	Expression is in smooth muscle in the blood vessels.

2_E11.5 CD31/SMA ©

Specimen 2 E11.5 CD31: embryonic day 11.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS19: yolk sac	Present	Regionally restricted	2_E11.5 CD31/SMA	Expression is in smooth muscle in the blood vessels.

2_E14.5 CD31/SMA ©

Specimen 2 E14.5 CD31: embryonic day 14.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS22: yolk sac	Present	Regionally restricted	2_E14.5 CD31/SMA	Expression is in smooth muscle in the blood vessels.

2_E18.5 CD31/SMA ©

Specimen 2 E18.5 CD31: embryonic day 18.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS26: yolk sac	Present	Regionally restricted	2_E18.5 CD31/SMA	Expression is in smooth muscle in the blood vessels.

4A ©

Specimen 4A: embryonic day 9.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Regionally restricted	4A	Expression is in smooth muscle.
TS15: dorsal aorta	Present	Regionally restricted	4A	Expression is in smooth muscle.

4B ©

Specimen 4B: embryonic day 9.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS15: cardinal vein	Present	Regionally restricted	4B	Expression is in smooth muscle.
TS15: dorsal aorta	Present	Regionally restricted	4B	Expression is in smooth muscle.

4E ©

Specimen 4E: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS17: cardinal vein	Present	Regionally restricted	4E	Expression is in smooth muscle.
TS17: dorsal aorta	Present	Regionally restricted	4E	Expression is in smooth muscle.

4F ©

Specimen 4F: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS17: cardinal vein	Present	Regionally restricted	4F	Expression is in smooth muscle.
TS17: dorsal aorta	Present	Regionally restricted	4F	Expression is in smooth muscle.

4I ©

Specimen 4I: embryonic day 9.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present	Regionally restricted	4I	Expression is in smooth muscle.

4K ©

Specimen 4K: embryonic day 9.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Present	Regionally restricted	4K	Expression is in smooth muscle.

4M ©

Specimen 4M: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	4M	Expression is in smooth muscle.

4N ©

Specimen 4N: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Nrp1 (assay MGI:5555946).

Structure	Level	Pattern	Image	Note
TS17: dorsal aorta	Present	Regionally restricted	4N	Expression is in smooth muscle.

4P ©

Specimen 4P: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Lyve1 (assay MGI:5555940); blue - Nrp1 (assay MGI:5555946).

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present	Regionally restricted	4P	Expression is in smooth muscle.

5A ©

Specimen 5A: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present		5A

5B ©

Specimen 5B: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS17: endocardial lining	Present		5B

5C ©

Specimen 5C: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS17: liver	Absent		5C

5D ©

Specimen 5D: embryonic day 10.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS17: liver	Absent		5D

5E ©

Specimen 5E: embryonic day 12.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	5E

5F ©

Specimen 5F: embryonic day 12.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	5F

5G ©

Specimen 5G: embryonic day 12.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Image
TS20: blood vessel	Present	5G
TS20: liver	Present	5G
TS20: lung	Present	5G

5H ©

Specimen 5H: embryonic day 12.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Image
TS20: blood vessel	Present	5H
TS20: liver	Present	5H
TS20: lung	Present	5H

5I ©

Specimen 5I: embryonic day 16.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS24: smooth muscle tissue	Present		5I

5J ©

Specimen 5J: embryonic day 16.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS24: smooth muscle tissue	Present		5J

5K ©

Specimen 5K: embryonic day 16.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Prox1 (assay MGI:5555924).

Structure	Level	Pattern	Image	Note
TS24: smooth muscle tissue	Present		5K

5L ©

Specimen 5L: embryonic day 16.5 (more

)
Note: Triple labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937); blue - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS24: smooth muscle tissue	Present		5L

Specimen S2 E11.5 LYVE-1: embryonic day 11.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS19: yolk sac	Present	Regionally restricted	S2_E11.5 SMA with LYVE-1	Expression is in smooth muscle in the blood vessels.

Specimen S2 E18.5 LYVE-1: embryonic day 18.5 (more

)
Note: Double labeled: red - Acta2; green - Lyve1 (assay MGI:5555940).

Structure	Level	Pattern	Image	Note
TS26: yolk sac	Present	Regionally restricted	S2_E18.5 SMA with LYVE-1	Expression is in smooth muscle in the blood vessels.

Specimen S2 E11.5 CD31: embryonic day 11.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS19: yolk sac	Present	Regionally restricted	S2_E11.5 SMA with CD31	Expression is in smooth muscle in the blood vessels.

Specimen S2 E18.5 CD31: embryonic day 18.5 (more

)
Note: Double labeled: red - Acta2; green - Pecam1 (CD31; assay MGI:5555937).

Structure	Level	Pattern	Image	Note
TS26: yolk sac	Present	Regionally restricted	S2_E18.5 SMA with CD31	Expression is in smooth muscle in the blood vessels.

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