Antibody:	anti-SPARC
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: brain ventricular layer	Present	Regionally restricted	1A	Expressed in parts of the ventricular zone in all brain vesicles.
TS17: telencephalon ventricular layer	Present		1A

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present		1B

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1C	Expressed in radial glia cells.

Structure	Level	Pattern	Image	Note
TS17: telencephalon ventricular layer	Present	Regionally restricted	1D	Expression is excluded from Dcx-positive immature neurons.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	1E	Expressed in the central canal. Expressed in radial glia cells.

Structure	Level	Pattern	Image	Note
TS19: brain ventricular layer	Strong	Regionally restricted	1G , 1F	Highly enriched in brain flexures. Expressed in radial glia cells.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		1I , 1J
TS17: telencephalon ventricular layer	Present		1I , 1J

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2A	Expressed in the hippocampal neuroepithelium. Expressed in the ventricular zone.
TS21: choroid plexus	Strong		2A
TS21: blood vessel	Strong		2A
TS21: lateral ganglionic eminence	Present		2A

Structure	Level	Pattern	Image	Note
TS21: cerebral aqueduct	Present	Regionally restricted	2B	Expressed in the neuroepithelium.
TS21: hindbrain	Present	Regionally restricted	2B	Expressed in the neuroepithelium.
TS21: cerebellum	Strong	Regionally restricted	2B	Expressed in discreet regions.
TS21: choroid plexus	Strong		2B
TS21: hindbrain pia mater	Strong		2B
TS21: blood vessel	Strong		2B

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2C	Expressed in the ventricular zone. Expressed by nestin-positive radial glia.
TS21: choroid plexus	Strong		2C

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	2D , 2E	Expressed in the ventricular zone. Overlaped with the BLBP-positive subpopulation of radial glia.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong	Regionally restricted	2F	Expressed in discreet regions. Overlaped with the BLBP-positive subpopulation of radial glia.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Strong	Regionally restricted	2G	Expressed in discreet regions. Expressed by nestin-positive radial glia.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Strong	Regionally restricted	2H	Expressed in discreet regions. Overlaped with the BLBP-positive subpopulation of radial glia.

Structure	Level	Pattern	Image	Note
TS25: brainstem	Strong	Regionally restricted	2I	Expressed in discreet regions. Expressed by nestin-positive radial glia.
TS25: blood vessel	Strong		2I

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2J	Expressed in the central canal and floorplate. Overlaped with the BLBP-positive subpopulation of radial glia.
TS21: spinal cord floor plate	Present		2J
TS21: blood vessel	Strong		2J

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2K	Expressed in the central canal and floorplate. Not expressed by neurons (betaIII tubulin-positive).
TS21: spinal cord floor plate	Present		2K

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	2L	Expressed in the central canal and floorplate. Not expressed by neuroblasts (Dcx-positive).
TS21: spinal cord floor plate	Present		2L

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present	Regionally restricted	2M	Expressed in the central canal and floorplate. Overlaped with the BLBP-positive subpopulation of radial glia.
TS25: spinal cord floor plate	Present		2M
TS25: blood vessel	Strong		2M

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present	Regionally restricted	2N	Expressed in the central canal and floorplate. Overlaped with the BLBP-positive subpopulation of radial glia.
TS25: spinal cord floor plate	Present		2N

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Strong	Regionally restricted	3A	Expressed in the cell bodies of radial glia lining the ventricles, and in their endfeet on the pial surface.
TS27: brain blood vessel	Strong		3A

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Strong	Regionally restricted	3B	Expressed in the cell bodies of radial glia lining the ventricles, and in their endfeet on the pial surface.
TS28: brain blood vessel	Strong		3B

Structure	Level	Pattern	Image	Note
TS28: brainstem	Strong		3B inset

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	3C	Expressed in radial glia in the rostral migratory stream, surrounding proliferating (PCNA-positive) migratory cells.
TS28: brain blood vessel	Strong		3C

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Strong	Regionally restricted	3D	Expressed in the cell bodies of radial glia lining the ventricles.
TS27: choroid plexus	Strong		3D

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	3D inset	Expressed in a subset of ADAM21-positive radial glia lining the dorsal wall.

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Strong	Regionally restricted	3E	Expressed in the cell bodies of radial glia lining the ventricles.

Structure	Level	Pattern	Image	Note
TS27: fimbria hippocampus	Strong	Regionally restricted	3F	Expressed in the cell bodies of radial glia lining the ventricles.

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	3G	Expressed in a small number of radial glia-like processes, extending from the lateral ventricle along the rostral migratory stream.
TS28: lateral ventricle ependyma	Weak		3G

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Weak		3G inset

Structure	Level	Pattern	Image	Note
TS28: rostral migratory stream	Present	Regionally restricted	3H	Expressed in a small number of radial glia-like processes, extending from the lateral ventricle along the rostral migratory stream.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Strong		3I	Expressed in radial glia cells.
TS27: dentate gyrus	Present		3I	Expressed in radial glia cells.
TS27: dentate gyrus molecular layer	Weak	Punctate	3I

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus molecular layer	Strong	Punctate	3I inset

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	3J	Expressed in radial glia cells.
TS28: dentate gyrus molecular layer	Strong	Punctate	3J	Not colocalises with nestin or BLBP.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	3K	Expressed in radial glia cells.
TS28: dentate gyrus molecular layer	Strong	Punctate	3K	Not colocalises with nestin or BLBP.
TS28: brain blood vessel	Strong		3K

Structure	Level	Pattern	Image	Note
TS27: 3rd ventricle ependyma	Present		3M

Structure	Level	Pattern	Image	Note
TS28: brain blood vessel	Strong		3N

Structure	Level	Pattern	Image	Note
TS28: brainstem	Present	Regionally restricted	3O	Expressed in the endfeet of the astrocytes surrounding blood vessels (part of the blood-brain barrier).

Structure	Level	Pattern	Image	Note
TS27: brain blood vessel	Present		4A
TS27: pia mater	Present		4A

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Punctate	4B	Expressed in Bergmann glia cells.
TS28: cerebellum Purkinje cell layer	Present	Punctate	4B	Expressed in Bergmann glia cells and Purkinje cell bodies.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	4C	Expressed in all Bergmann glia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	4D	Expressed in all Bergmann glia, with particular intensity in their cell bodies and endfeet on the pial surface.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	4E	Expressed in all Bergmann glia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong	Regionally restricted	4F	Expressed in all Bergmann glia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		4G	Expressed in microglia.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	4H	Not expressed by neurons, including Purkinje cells.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	4I	Expressed in the endfeet of Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	4J	Expressed in cell bodies and endfeet of Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	4K right , 4K left	Expressed in cell bodies and endfeet of Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	4L	Expressed in Muller glia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present		4M	Expressed in Muller glia and microglia.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	4N	Expressed in Muller glia, but not in neuroblasts.

Structure	Level	Pattern	Image	Note
TS28: retina	Present	Regionally restricted	4O	Expressed in Muller glia, but not in neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5D	Expressed exclusively in microglia.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		5E	Expressed exclusively in microglia, but not in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: pons	Present		5I	Expressed in astrocytes.

Structure	Level	Pattern	Image	Note
TS28: spinal cord	Present	Regionally restricted	5J	Expressed in astrocytes in the grey matter.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		5L , 5M , 5K	Expressed in microglia, but not in the macrophage in the olfactory nerve. Expression in astrocytes is little or absent.

Structure	Level	Pattern	Image	Note
TS21: cranial nerve	Strong		6A	Expressed in Schwann cells.

Structure	Level	Pattern	Image	Note
TS21: facial nerve	Strong		6B	Expressed in Schwann cells.

Structure	Level	Pattern	Image	Note
TS21: facial nerve	Strong		6D , 6C	Expressed in Schwann cells. Expression is concentrated in the cell soma.

Structure	Level	Pattern	Image	Note
TS28: facial nerve	Strong		6F , 6E	Expressed in Schwann cells, including around nodes of Ranvier.

Structure	Level	Pattern	Image	Note
TS28: sciatic nerve	Strong		6G	Expressed in Schwann cells. Expression is distributed along the outer plasma membrane and/or basal lamina.

Structure	Level	Pattern	Image	Note
TS21: olfactory nerve	Strong		6H	Expressed in the olfactory ensheathing cells.

Structure	Level	Pattern	Image	Note
TS21: olfactory nerve	Strong		6I	Expressed in the olfactory ensheathing cells.

Structure	Level	Pattern	Image	Note
TS21: olfactory nerve	Present		6I inset	Only expressed in the glial processes that surround the outside of olfactory axon bundles.

Structure	Level	Pattern	Image	Note
TS28: olfactory nerve	Weak	Regionally restricted	6J	Expressed in the glial processes that surround mesaxons.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Regionally restricted		Expressed in a small number of radial glia-like processes, extending from the lateral ventricle into the striatum.
TS28: corpus striatum	Present	Regionally restricted		Expressed in a small number of radial glia-like processes, extending from the lateral ventricle into the striatum.
TS28: telencephalon	Present	Regionally restricted		Expressed in a small number of radial glia-like processes, extending from the lateral ventricle into the striatum.

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Strong			Expressed in spinal nerve roots and cauda equina.