Gene Expression Data
Assay Details
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Assay
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Reference:
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J:124197
Kiyozumi D, et al., Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1. Matrix Biol. 2007 Jul;26(6):456-462
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5578504
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Gene symbol:
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Frem3
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Gene name:
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Fras1 related extracellular matrix protein 3
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Antibody:
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anti-Frem3 NV domain
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Cryosections were fixed in absolute ethanol.
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Results
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Specimen
2B:
postnatal week 9
(more  )
Note: Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
Specimen
2B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 9
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
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Specimen
2C:
embryonic day 14.5
(more )
Note: Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
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Specimen
2D:
postnatal adult
(more )
Note: Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
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Specimen
2E:
embryonic day 14.5
(more )
Note: Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: metanephros |
Present |
Regionally restricted |
2E Frem3
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Expression is in the renal epithelial basement membrane.
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Specimen
3A:
embryonic day 17.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: epidermis |
Present |
Regionally restricted |
3A Frem3
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Expression is at the interfollicular epidermal basement membrane.
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| TS25: hair follicle |
Present |
Regionally restricted |
3A Frem3
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Expression is at the stalk region with a descending proximal-distal gradient.
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Specimen
3B:
postnatal week 4
(more )
Note: Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal week 4
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Frem3; red - Lamc1 (assay MGI:5578519).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epidermis |
Present |
Regionally restricted |
3B Frem3
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Expression is at the interfollicular epidermal basement membrane.
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| TS28: coat hair follicle |
Present |
Regionally restricted |
3B Frem3
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Expression is at the basement membrane engulfing the dermal papilla.
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| TS28: hair outer root sheath |
Present |
Regionally restricted |
3B Frem3
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Expression is at the basement membrane.
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Specimen
4A Qbrick/Frem1+/-:
postnatal newborn; Frem1tm1Ksek/Frem1 +
(more )
Specimen
4A Qbrick/Frem1+/-:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Frem1tm1Ksek/Frem1+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Qbrick/Frem1+/- Frem3
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Expression is at the basement membrane.
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Specimen
4A Qbrick/Frem1-/-:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Frem1tm1Ksek/Frem1tm1Ksek
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Qbrick/Frem1-/- Frem3
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Expression is at the basement membrane and is the same as in wild-type.
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Specimen
4A Frem2my/+:
postnatal newborn; Frem2my/Frem2 +
(more )
Specimen
4A Frem2my/+:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Frem2my/Frem2+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Frem2my/+ Frem3
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Expression is at the basement membrane.
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Specimen
4A Frem2my/my:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Frem2my/Frem2my
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Frem2my/my Frem3
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Expression is at the basement membrane and is the same as in wild-type.
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Specimen
4A Grip1eb/+:
postnatal newborn; Grip1eb/Grip1 +
(more )
Specimen
4A Grip1eb/+:
(close )
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Genetic Background:
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involves: hairless stock
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Mutant Allele(s):
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Grip1eb/Grip1+
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Grip1eb/+ Frem3
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Expression is at the basement membrane.
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Specimen
4A Grip1eb/eb:
(close )
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Genetic Background:
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involves: hairless stock
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Mutant Allele(s):
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Grip1eb/Grip1eb
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: epidermis |
Present |
Regionally restricted |
4A Grip1eb/eb Frem3
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Expression is at the basement membrane and is the same as in wild-type.
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Specimen
4B Frem2my/+:
embryonic day 13.5; Frem2my/Frem2 +
(more )
Specimen
4B Frem2my/+:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Frem2my/Frem2+
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: epidermis |
Present |
Regionally restricted |
4B Frem2my/+ Frem3
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Expression is at the basement membrane.
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Specimen
4B Frem2my/my:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Frem2my/Frem2my
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: epidermis |
Present |
Regionally restricted |
4B Frem2my/my Frem3
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Expression is at the basement membrane and is the same as in wild-type.
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Specimen
4C Grip1eb/+:
embryonic day 14.5; Grip1eb/Grip1 +
(more )
Specimen
4C Grip1eb/+:
(close )
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Genetic Background:
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involves: hairless stock
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Mutant Allele(s):
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Grip1eb/Grip1+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Present |
Regionally restricted |
4C Grip1eb/+ Frem3
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Expression is at the basement membrane.
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Specimen
4C Grip1eb/eb:
(close )
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Genetic Background:
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involves: hairless stock
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Mutant Allele(s):
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Grip1eb/Grip1eb
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: epidermis |
Present |
Regionally restricted |
4C Grip1eb/eb Frem3
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There was a slight reduction in expression in this mutant.
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