Antibody:	malE-SCL antibody
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		2A
TS11: extraembryonic mesoderm	Present		2A

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		2B	Expression is in both centrally located hematopoietic cells and peripherally placed endothelial cells.
TS13: blood	Present		2B	Expression is in nucleated blood cells in vascular structures.

Structure	Level	Pattern	Image	Note
TS15: hepatic primordium	Present		2C
TS15: neural tube	Present	Regionally restricted	2C	Expression is in the ventral wall.

Structure	Level	Pattern	Image	Note
TS20: liver	Very strong		2D

Structure	Level	Pattern	Image	Note
TS20: blood	Very strong		2F
TS20: liver	Very strong		2F

Structure	Level	Pattern	Image	Note
TS23: spleen primordium	Present		2G

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	2H	Expression is in the subependymal cells.

Structure	Level	Pattern	Image	Note
TS22: axial skeleton	Present	Regionally restricted	2I	Expression is in the perichondrium of the vertebrea.

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		2J top panel

Structure	Level	Pattern	Image	Note
TS28: spleen	Present		2K	Expression is in a megakaryocyte.

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Present	Single cells	2L	Expression is in small numbers of mononuclear cells.
TS28: thymus medullary core	Present	Single cells	2L	Expression is in small numbers of mononuclear cells.

Structure	Level	Pattern	Image	Note
TS13: blood island	Present		3A	Expression is in hematopoietic cells and endothelial cells.

Structure	Level	Pattern	Image	Note
TS21: lung vascular element	Present	Regionally restricted	3B	Expression is in angioblasts.

Structure	Level	Pattern	Image	Note
TS23: lung vascular element	Present	Regionally restricted	3C	Expression is in endothelial celss of small vessels.

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		3D
TS20: endocardial lining	Present		3D

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Present		3E

Structure	Level	Pattern	Image	Note
TS23: blood vessel	Present	Regionally restricted	3F	Expression is in endothelial cells of pulmonary vessels.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		3G	Expression is in the endothelial cells lining the blood sinuses.

Structure	Level	Pattern	Image	Note
TS13: future brain neural fold	Present	Regionally restricted		The authors state that starting at E8.5-E9.5 expression is at the rostral and caudal ends of the closing neural tube.
TS13: future spinal cord neural fold	Present	Regionally restricted		The authors state that starting at E8.5-E9.5 expression is at the rostral and caudal ends of the closing neural tube.

Structure	Level	Pattern	Image	Note
TS15: blood vessel	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.
TS15: branchial arch	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.
TS15: optic cup	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.

Structure	Level	Pattern	Image	Note
TS17: liver	Strong
TS17: dorsal aorta	Present
TS17: endocardial lining	Present

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.
TS19: branchial arch	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.
TS19: retina	Present	Regionally restricted		The authors state that expression was seen in endothelial cells in the retina and branchial arches from days E9.5-E11.5.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present
TS21: dorsal aorta	Present
TS21: endocardial tissue	Present
TS21: metanephros	Present
TS21: intestine	Present	Regionally restricted		Expression is in the submucosa.

Structure	Level	Pattern	Image	Note
TS22: liver	Strong
TS22: nasal cavity epithelium	Present	Regionally restricted		Expression is in the sensory epithelial cells.
TS22: external auditory meatus	Present	Regionally restricted		Expression is in the columnar epithelial cells.
TS22: lung	Present
TS22: metanephros	Present
TS22: femur cartilage condensation	Present	Regionally restricted		Expression is in the periosteal layer.
TS22: rib cartilage condensation	Present	Regionally restricted		Expression is in the periosteal layer.
TS22: cochlea	Present	Regionally restricted		Expression is in cartilage.
TS22: maxilla	Present	Regionally restricted		Expression is in cartilage.
TS22: trachea	Present	Regionally restricted		Expression is in cartilage.
TS22: cranium	Present	Regionally restricted		Expression is in membranous bone.

Structure	Level	Pattern	Image	Note
TS23: aorta	Present	Regionally restricted		Expression is in endothelial cells.
TS23: dermis	Present
TS23: metanephros	Present
TS23: salivary gland	Present
TS23: intestine	Present	Regionally restricted		Expression is in the submucosa.
TS23: femur	Present	Regionally restricted		Expression is in the periosteal layer.
TS23: rib	Present	Regionally restricted		Expression is in the periosteal layer.
TS23: thymus primordium	Present	Single cells		Expression is in small numbers of mononuclear cells.

Structure	Level	Pattern	Image	Note
TS25: salivary gland	Present

Structure	Level	Pattern	Image	Note
TS27: liver	Weak
TS27: spleen	Present			The authors state that expression was detected in the spleen starting at E15.5 through birth.
TS27: aorta	Present	Regionally restricted		Expression is in smooth muscle.
TS27: bladder	Present	Regionally restricted		Expression is in smooth muscle.

Structure	Level	Pattern	Image	Note
TS27: liver	Weak

Structure	Level	Pattern	Image	Note
TS28: liver	Weak

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present
TS28: thalamus	Present	Single cells
TS28: heart	Absent
TS28: liver	Absent
TS28: lung	Absent
TS28: metanephros	Absent
TS28: pituitary gland	Absent
TS28: thyroid gland	Absent