Antibody:	anti-Iba1
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS17: central nervous system	Strong	Scattered	3A' , 3A''	Expression was detected in mononuclear phagocytes and co-localized with Dab2.
TS17: embryo mesenchyme	Present		3A'' , 3A'	Expression was in fetal macrophages and co-localized with Dab2.

Structure	Level	Pattern	Image	Note
TS20: central nervous system	Present	Scattered	3C	Expression was in microglial progenitors. These were not Dab2 immunopositive.
TS20: embryo mesenchyme	Present		3C	Expression was in fetal macrophages and co-localized with Dab2.

Structure	Level	Pattern	Image	Note
TS22: embryo mesenchyme	Present		3H' , 3H''	Expression was in macrophages in the mesenchyme and co-localized with Dab2.
TS22: central nervous system	Present		3H' , 3H''	The immunopositive microglial progenitor cells found dispersed in the neural tube did not co-localize Dab2.

Structure	Level	Pattern	Image	Note
TS15: head mesenchyme	Present		3N' , 3N''	Expression co-localized with Dab2-positive macrophages.