Gene Expression Data
Assay Details
Assay
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Reference:
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J:51432
Mallamaci A, et al., EMX2 protein in the developing mouse brain and olfactory area. Mech Dev. 1998 Oct;77(2):165-72
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5648402
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Gene symbol:
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Emx2
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Gene name:
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empty spiracles homeobox 2
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Antibody:
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Anti-Emx2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1a:
embryonic day 12.0
(more )
Specimen
1a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Graded |
1a
|
Expression restricted mainly to telencephallic neuroepithelium. Distribution is graded along anterior-posterior axis, with a caudal-medial maximum and rostral-lateral minimum.
|
TS20: cerebral cortex marginal layer |
Present |
Scattered |
1a
|
Intense expression in a few scattered cells in the marginal-most part of the cerebral wall, more abundant at its caudal pole
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Specimen
1b:
embryonic day 15.0
(more )
Specimen
1b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex |
Present |
Graded |
1b
|
Distribution of expression in telencephallic neuroepithelium is graded along anterior-posterior axis, with a caudal-medial maximum and rostral-lateral minimum.
|
TS23: cerebral cortex ventricular layer |
Present |
|
1b
|
Expression mainly confined to ventricular zone. Expression in ventricular neuroepithelium.
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TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
1b
|
Expression in scattered cells in marginal layer. Expression still abundant in cells in marginal-most part of cerebral wall. Faint expression in marginal-most part of the forming cortical plate.
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TS23: cortical plate |
Absent |
|
1b
|
Expression absent in transitional field, subplate and cortical plate.
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TS23: cortical subplate |
Absent |
|
1b
|
Expression absent in transitional field, subplate and cortical plate.
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TS23: hippocampus |
Present |
|
1b
|
Expression in hippocampal anlage.
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TS23: telencephalon |
Strong |
Regionally restricted |
1b
|
Intense expression in numerous cells underneath the pia, in area of secondary proliferative zone for dentate gyrus.
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Specimen
1c:
embryonic day 18.0
(more )
Specimen
1c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: occipital cortex |
Present |
Regionally restricted |
1c
|
Expression in parieto-occipital cerebral cortex.
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TS26: parietal cortex |
Present |
Regionally restricted |
1c
|
Expression in parieto-occipital cerebral cortex.
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TS26: cerebral cortex ventricular zone |
Present |
Graded |
1c
|
Expression mainly confined to ventricular zone. Higher expression in single ventricular zones in the caudal-medial than in rostral-lateral telencephalon.
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TS26: cerebral cortex marginal zone |
Present |
Scattered |
1c
|
Expression in scattered cells at outer border of marginal layer.
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TS26: neocortex |
Present |
Regionally restricted |
1c
|
Expression in outer part of neocortical layer I, in an immediately subpial position.
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Specimen
1d:
embryonic day 15.0
(more )
Specimen
1d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
1d
|
Expression restricted to scattered cells in outer marginal layer
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TS23: cortical plate |
Present |
Regionally restricted |
1d
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Expression specifically localized in proximal pieces of apical dendrite of outer cortical plate neurons.
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Specimen
1d':
embryonic day 15.0
(more )
Note: Double labeled: brown - Emx2; black - Gap43 (assay MGI:5648404)
Specimen
1d':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: brown - Emx2; black - Gap43 (assay MGI:5648404)
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
1d'
|
Expression restricted to scattered cells in outer marginal layer
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TS23: cortical plate |
Present |
Regionally restricted |
1d'
|
Expression specifically localized in proximal pieces of apical dendrite of outer cortical plate neurons.
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Specimen
1e:
embryonic day 18.0
(more )
Note: Double labeled: brown - Emx2; black - Calb2 (assay MGI:5648406)
Specimen
1e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: brown - Emx2; black - Calb2 (assay MGI:5648406)
|
Specimen
1f:
embryonic day 18.0
(more )
Specimen
1f:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
Regionally restricted |
1f
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Distribution of expression in telecephallic neuroepithelium is graded along medial-lateral axis, with a caudal-medial maximum and rostral-lateral minimum.
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TS26: amygdala |
Present |
Regionally restricted |
1f
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Expression in medial part of nuclear complex of amygdala.
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Specimen
1g:
embryonic day 15.0
(more )
Specimen
1g:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: main olfactory bulb |
Present |
Regionally restricted |
1g
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Abundant expression in mitral layer of accessory olfactory bulb. Expression in numerous cells in the subependymal layer; subependymal domain stops at boundary between bulbar and retrobulbar zones. Low level of expression in proliferative layer.
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Specimen
1h:
embryonic day 18.0
(more )
Specimen
1h:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
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Specimen
1i:
postnatal day 5
(more )
Specimen
1i:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 5
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Sex:
|
Not Specified
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Type:
|
section
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Specimen
2a:
embryonic day 9.5
(more )
Specimen
2a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future midbrain |
Present |
Regionally restricted |
2a
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Expression in a few marginally located cells in the ventral mesencephalon.
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Specimen
2b:
embryonic day 18.0
(more )
Specimen
2b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: midbrain tegmentum |
Present |
Regionally restricted |
2b
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Expression in ventral midbrain in a group of cells in medial part of developing tegmentum.
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Specimen
2c:
embryonic day 12.5
(more )
Specimen
2c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Strong |
Regionally restricted |
2c
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Intense expression in the mammillary area.
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TS20: midbrain |
Present |
Regionally restricted |
2c
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Enlarged expression domain, extended rostro-caudally along almost all of midbrain.
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TS20: midbrain roof plate |
Present |
Regionally restricted |
2c
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Expression in a cohort of cells located in an intermediate position between ventricular and marginal edges of dorsal mesencephalic wall. Expression in a row of cells throughout the entire developing tectum.
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Specimen
2d:
embryonic day 18.0
(more )
Note: Double labeled: dark brown - Emx2; light blue - Calb1 (assay MGI:5648407)
Specimen
2d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Note:
|
Double labeled: dark brown - Emx2; light blue - Calb1 (assay MGI:5648407)
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: tectum |
Present |
Scattered |
2d
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Expression detected in three deeper longitudinal stripes. Expression in scattered cells located in all 3 presumptive strata grisea of the tectum.
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Specimen
3a:
embryonic day 9.0
(more )
Specimen
3a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section
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Specimen
3b:
embryonic day 10.5
(more )
Specimen
3b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
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Type:
|
section
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Specimen
3c:
embryonic day 12.0
(more )
Specimen
3c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
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Structure |
Level |
Pattern |
Image |
Note |
TS20: head mesenchyme |
Present |
Regionally restricted |
3c
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Expression detected in scattered cells located in mesenchyme between olfactory pit and telencephalic vesicle.
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TS20: telencephalon |
Present |
Regionally restricted |
3c
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Expression in two rows of cells on both sides of boundary between mesenchyme and telencephalic walls, along both alar and basal surfaces of telencephalic vesicle.
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Specimen
3d:
embryonic day 12.5
(more )
Specimen
3d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: olfactory epithelium |
Present |
|
3d
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Expression in precursors of both main olfactory epithelium and vomeronasal organ, just budding off enlarging nasal cavity.
|
TS20: vomeronasal organ epithelium |
Present |
|
3d
|
Expression in precursors of both main olfactory epithelium and vomeronasal organ, just budding off enlarging nasal cavity.
|
TS20: head mesenchyme |
Present |
Regionally restricted |
3d
|
Expression in scattered cells in mesenchyme between main olfactory epithelium and vomeronasal organ.
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Specimen
3e:
embryonic day 16.5
(more )
Specimen
3e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: olfactory epithelium |
Present |
Regionally restricted |
3e
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Expression in main olfactory epithelium restricted to nuclei located in the outer half of epithelium, possibly basal cells (olfactory epithelium stem cells) and young sensory neurons. No expression in mature neurons located in luminal half of epithelium.
|
Specimen
E8.5 not shown:
embryonic day 8.5
(more )
Specimen
E8.5 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: head |
Present |
Regionally restricted |
|
Expression in anterior headfold, more intense laterally than medially.
|
Specimen
E9.5 not shown:
embryonic day 9.5
(more )
Specimen
E9.5 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
|
Expression in the mammillary area.
|
Specimen
E10 not shown:
embryonic day 10.0
(more )
Specimen
E10 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: telencephalon |
Present |
Regionally restricted |
|
Expression in presumptive cerebral cortex, throughout entire width of telencephalic wall, more abundant dorso-caudally than ventro-rostrally. Almost undetectable in developing ganglionic eminence.
|
Specimen
E12.5 not shown:
embryonic day 12.5
(more )
Specimen
E12.5 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
|
Expression only a few scattered cells in the first rhombomere.
|
Specimen
E15 not shown:
embryonic day 15.0
(more )
Specimen
E15 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
|
Expression in numerous cells in the area of basal telencephalon.
|
Specimen
E16.5 not shown:
embryonic day 16.5
(more )
Specimen
E16.5 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Specimen
E18 not shown:
embryonic day 18.0
(more )
Specimen
E18 not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: main olfactory bulb |
Present |
Regionally restricted |
|
Expression in presumptive interneurons and glial cells of the internal granular layer.
|
Specimen
postnatal not shown:
postnatal
(more )
Specimen
postnatal not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
|