Antibody:	anti-NH-Prox1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: prethalamus	Absent		3B	There is no protein expression of in the area of the prethalamus (but there is mRNA present).
TS24: pretectum	Present		3B
TS24: pons	Present		3B
TS24: tectum	Present		3B	Expression in the colliculus.
TS24: epithalamus	Present		3B
TS24: midbrain tegmentum	Present		3B

Structure	Level	Pattern	Image	Note
TS24: prethalamus	Absent		3D	There is no protein expression of in the area of the prethalamus (but there is mRNA present).

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Present	Regionally restricted	3D inset	Expression is present in the dentate migratory stream.

Structure	Level	Pattern	Image	Note
TS24: thalamus	Present	Regionally restricted	3E	Areas of expression include the paraventricular nucleus.
TS24: prethalamus	Absent		3E	There is no protein expression of in the area of the prethalamus (but there is mRNA present).
TS24: amygdala	Present	Regionally restricted	3E	Expression is present in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS24: epithalamus	Present		3G
TS24: thalamus	Present		3G	Areas of expression include the dorsal thalamus.
TS24: hypothalamus	Present	Regionally restricted	3G	Expression is present in the dorsomedial hypothalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present		3G inset

Structure	Level	Pattern	Image	Note
TS24: amygdala	Present	Regionally restricted	3H	Expression is present in the subventricular zone.
TS24: hypothalamus	Present	Regionally restricted	3H	Expression is present in the ventromedial hypothalmus.
TS24: thalamus	Present	Regionally restricted	3H	Although there is protein expression elsewhere in the thalamus, there is no expression in the subgeniculate nucleus.
TS24: zona incerta	Absent		3H	There is no expression in the ventral portion of the zona incerta. This area does have mRNA expression.

Structure	Level	Pattern	Image	Note
TS24: pons	Present		3K	Areas of expression include the pontine nucleus.
TS24: neurohypophysis pars nervosa	Present		3K	Expression in the posterior lobe of the pituitary.

Structure	Level	Pattern	Image	Note
TS24: medulla oblongata	Present

Structure	Level	Pattern	Image	Note
TS26: thalamus	Strong	Regionally restricted	4B	Areas of expression include the laterodorsal and ventrolateral thalamus.
TS26: prethalamus	Absent		4B	There is no expression present in the area surrounding the prethalamus.
TS26: dentate gyrus	Present		4B

Structure	Level	Pattern	Image	Note
TS26: thalamus	Strong	Regionally restricted	4D	Although protein is present elsewhere in the thalamus, it is not found in the subgeniculate nucleus.
TS26: zona incerta	Absent		4D	No expression in the ventral zona incerta.
TS26: dentate gyrus	Present		4D

Structure	Level	Pattern	Image	Note
TS26: thalamus	Strong	Regionally restricted	4F	Protein expression is present in the posterior nucleus of thalamus.
TS26: dentate gyrus	Present		4F

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		4G
TS26: cerebral cortex marginal zone	Present		4G	Expression is present along the mantle zone.

Structure	Level	Pattern	Image	Note
TS26: tectum	Present	Regionally restricted	4H	Expression is present along the mantle zone of the colliculus, as well as the subventricular zone.