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Gene Expression Data
Assay Details
Assay
Reference: J:211312 Woo SH, et al., Piezo2 is required for Merkel-cell mechanotransduction. Nature. 2014 May 29;509(7502):622-6
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5697548
Gene symbol: Nefh
Gene name: neurofilament, heavy polypeptide
Antibody: anti-Nefh (AB8135)
Detection system: Secondary antibody
Results
Specimen Ext. 2A: postnatal (more )
Note: whisker pad; double labeled: blue - Nefh; red - Krt8 (assay MGI:5697549).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Regionally restricted Extended Data 2A Merge , Extended Data 2A Nefh Expression is present in sensory afferents, including those that innervate Merkel cells.
Specimen Ext. 2D: postnatal; Piezo2tm1.1(cre)Apat/Piezo2tm1.1(cre)Apat (more )
Note: whisker pad; double labeled: red - Nefh; green - Piezo2 reporter (assay MGI:5697408).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Regionally restricted Extended Data 2D , Extended Data 2F , Extended Data 2H Expression is present in the sensory afferents, including those that innervate Merkel cells.
Specimen 1C/D: postnatal; Piezo2tm1.1(cre)Apat/Piezo2tm1.1(cre)Apat (more )
Note: whisker pad; triple labeled: blue - Nefh; green - Piezo2 reporter (assay MGI:5697408); red - Krt8 (assay MGI:5697549).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Regionally restricted 1D GFP/Krt8/Nefh , 1D Nefh , 1C Nefh , 1C GFP/Krt8/Nefh Expression is present in the sensory afferents, including those that innervate Merkel cells.
Specimen 2F: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. whisker pad; triple labeled: blue - Nefh; green - Pizeo2 (assay MGI:5697547); red - Krt8 (assay MGI:5697549).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Regionally restricted 2F Nefh , 2F Krt8/Piezo2/Nefh Expression is present in the sensory afferents, including those that innervate Merkel cells.
Specimen Ext. 4E: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: dorsal skin; double labeled: green - Nefh; red - Krt8 (assay MGI:5697549).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4E Merge , Extended Data 4E Nefh Expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4F/G: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: dorsal skin; triple labeled: green - Nefh; red - Krt8 (assay MGI:5697549); blue - Slc17a6 (assay MGI:5697836).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4F Nefh , Extended Data 4G Merge , Extended Data 4G Krt8/Nefh Expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4H: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: dorsal skin; double labeled: green - Nefh; red - S100b (assay MGI:5697832).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4H Nefh , Extended Data 4H Merge Expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4I: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat (more )
Note: foot pad; double labeled: green - Nefh; red - S100b (assay MGI:5697832).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4I Merge , Extended Data 4I Nefh Expression is present in neurites in Merkel cell-neurite complexes in glabrous (nonhairy) skin.
Specimen Ext. 4J: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. dorsal skin; double labeled: green - Nefh; red - Krt8 (assay MGI:5697549).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4J Merge , Extended Data 4J Nefh As in wild type, expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4L: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. dorsal skin; triple labeled: green - Nefh; red - Krt8 (assay MGI:5697549); blue - Slc17a6 (assay MGI:5697836).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4L Krt8/Nefh , Extended Data 4L Merge As in wild type, expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4M: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. dorsal skin; double labeled: green - Nefh; red - S100 (assay MGI:5697832).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4M Nefh , Extended Data 4M Merge As in wild type, expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4N: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. dorsal skin; double labeled: green - Nefh; red - S100 (assay MGI:5697832).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4N Merge , Extended Data 4N Nefh As in wild type, expression is present in neurites in Merkel cell-neurite complexes in touch domes in hairy skin.
Specimen Ext. 4O: postnatal; Piezo2tm2.2Apat/Piezo2tm2.2Apat, Tg(KRT14-cre)1Amc/0 (more )
Note: Conditional mutant. foot pad; double labeled: green - Nefh; red - S100 (assay MGI:5697832).
Structure Level Pattern Image Note
TS28: peripheral nervous system Present Extended Data 4O Nefh , Extended Data 4O Merge As in wild type, expression is present in neurites in Merkel cell-neurite complexes in glabrous (nonhairy) skin.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
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