Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:99710
Kudo C, et al., Expression profiles of EphA3 at both the RNA and protein level in the developing mammalian forebrain. J Comp Neurol. 2005 Jul 4;487(3):255-69
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5699587
|
|
Gene symbol:
|
L1cam
|
|
Gene name:
|
L1 cell adhesion molecule
|
|
Antibody:
|
anti-NCAM L1 (clone 324)
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to rhodamine
|
|
|
Results
|
Specimen
9B/C/Q:
embryonic day 14.5
(more  )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
9B/C/Q:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
Specimen
9F/G/R:
embryonic day 14.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
9F/G/R:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
Specimen
9J/K/S:
embryonic day 16.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
9J/K/S:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
Specimen
9N/O/T:
embryonic day 18.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
9N/O/T:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
Specimen
10B/C:
embryonic day 14.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
10B/C:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
10B
,
10C
|
Coexpressed with Epha3 in corpus callosum.
|
Specimen
10E/F:
embryonic day 16.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
10E/F:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
Specimen
10H/I:
embryonic day 18.5
(more )
Note: Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
Specimen
10H/I:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: purple - L1cam; green - Epha3 (assay MGI:5699568).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: telencephalon |
Present |
Regionally restricted |
10I
,
10H
|
Coexpressed with Epha3 in corpus callosum.
|
Specimen
not shown:
embryonic day 14.5
(more )
Note: Also stained for Epha3 (assay MGI:5699568).
Specimen
not shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Also stained for Epha3 (assay MGI:5699568).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: telencephalon |
Present |
Regionally restricted |
|
Coexpressed with Epha3 in internal capsule.
|
| TS22: olfactory cortex |
Present |
Regionally restricted |
|
Coexpressed with Epha3 in lateral olfactory tract.
|
| TS22: diencephalon |
Present |
Regionally restricted |
|
Coexpressed with Epha3 in anterior commissure.
|
Specimen
not shown:
embryonic day 16.5
(more )
Note: Also stained for Epha3 (assay MGI:5699568).
Specimen
not shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Also stained for Epha3 (assay MGI:5699568).
|
Specimen
not shown:
embryonic day 18.5
(more )
Note: Also stained for Epha3 (assay MGI:5699568).
Specimen
not shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Also stained for Epha3 (assay MGI:5699568).
|
|
Analysis Tools