Gene Expression Data
Assay Details
Assay
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Reference:
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J:94531
Nieto M, et al., Expression of Cux-1 and Cux-2 in the subventricular zone and upper layers II-IV of the cerebral cortex. J Comp Neurol. 2004 Nov 8;479(2):168-80
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5702595
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Gene symbol:
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Cux1
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Gene name:
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cut-like homeobox 1
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Antibody:
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anti-Cux1
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Detection system:
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Alexa Fluor 488
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Results
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Specimen
1C:
postnatal adult
(more )
Specimen
1C:
(close )
Genetic Background:
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Swiss Webster
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Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
1C
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Expression restricted to upper layers. Expression in the majority of cells of layers II-IV, but only in a few cells of the deep layers.
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TS28: cortical layer II |
Present |
Regionally restricted |
1C
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Expression in neurons with pyramidally shaped cell bodies.
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TS28: cortical layer III |
Present |
Regionally restricted |
1C
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Expression in neurons with pyramidally shaped cell bodies.
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TS28: cortical layer IV |
Present |
Regionally restricted |
1C
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Expression in neurons with pyramidally shaped cell bodies.
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TS28: cerebral cortex marginal zone |
Absent |
|
1C
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Authors state that all cells of the marginal zone are negative for staining.
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Specimen
1C inset:
postnatal adult
(more )
Specimen
1C inset:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: telencephalon |
Present |
Regionally restricted |
1C inset
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Authors report nuclear localization of protein.
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Specimen
2A,C:
postnatal adult
(more )
Note: Double labeled: green - Cux1; red - Rbfox3 (assay MGI:5702596).
Specimen
2A,C:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Cux1; red - Rbfox3 (assay MGI:5702596).
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Specimen
2D:
postnatal adult
(more )
Note: Double labeled: green - Cux1; red - Rbfox3 (assay MGI:5702596).
Specimen
2D:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cux1; red - Rbfox3 (assay MGI:5702596).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
2D
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Expression in layers II-IV in neurons with pyramidally shaped cell bodies. No expression in some subpopulations of upper cortical neurons.
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Specimen
2E,G:
postnatal adult
(more )
Note: Double labeled: green - Cux1; red - Pvalb (assay MGI:5702597).
Specimen
2E,G:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Cux1; red - Pvalb (assay MGI:5702597).
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Specimen
2H:
postnatal adult
(more )
Note: Double labeled: green - Cux1; red - Pvalb (assay MGI:5702597).
Specimen
2H:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cux1; red - Pvalb (assay MGI:5702597).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
2H
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Authors report no expression in parvalbumin-expressing cells.`
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Specimen
3A:
postnatal day 21
(more )
Specimen
3A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3C:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Relnrl/Relnrl
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
3C
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Expression in late-born neurons located in the deeper cortex and do not form strict laminar boundaries.
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Specimen
4A:
embryonic day 13.5
(more )
Specimen
4A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex subventricular zone |
Weak |
|
4A
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Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
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TS21: cerebral cortex ventricular layer |
Weak |
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4A
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Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
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TS21: cortical plate |
Present |
Single cells |
4A
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Expression only in a few cells below the marginal zone in the upper most part of the cortical plate. No expression in cells destined to form the deep cortical layers.
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TS21: meninges |
Present |
Regionally restricted |
4A
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Authors report expression in cells of the pia, outside of the marginal zone.
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Specimen
4A inset:
embryonic day 13.5
(more )
Note: Double labeled: green - Cux1; red - Reln (assay MGI:5702598).
Specimen
4A inset:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cux1; red - Reln (assay MGI:5702598).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cortical plate |
Present |
Regionally restricted |
4A inset
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Expression only in a few cells below the marginal zone in the upper most part of the cortical plate.`
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Specimen
4B:
embryonic day 15.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B:
embryonic day 15.5
(more )
Note: Double labeled: green - Cux1; red - BrdU.
Specimen
5B:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Cux1; red - BrdU.
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Specimen
5C (top) VZ:
embryonic day 15.5
(more )
Note: Double labeled: green - Cux1; red - BrdU.
Specimen
5C (top) VZ:
(close )
Genetic Background:
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Swiss Webster
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Age:
|
embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Cux1; red - BrdU.
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Specimen
5C (middle) SVZ:
embryonic day 15.5
(more )
Note: Double labeled: green - Cux1; red - BrdU.
Specimen
5C (middle) SVZ:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cux1; red - BrdU.
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Specimen
5C (bottom):
embryonic day 15.5
(more )
Note: Double labeled: green - Cux1; red - BrdU.
Specimen
5C (bottom):
(close )
Genetic Background:
|
Swiss Webster
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
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Double labeled: green - Cux1; red - BrdU.
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Specimen
8A:
embryonic day 18.5
(more )
Specimen
8A:
(close )
Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8B:
(close )
Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
8C:
embryonic day 15.5
(more )
Specimen
8C:
(close )
Genetic Background:
|
Swiss Webster
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Age:
|
embryonic day 15.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
8D:
(close )
Genetic Background:
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involves: C57BL/6J * DBA/2J
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Mutant Allele(s):
|
Pax6Sey/Pax6Sey
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
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