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Gene Expression Data
Assay Details
Assay
Reference: J:169043 Chapman G, et al., Notch inhibition by the ligand Delta-Like 3 defines the mechanism of abnormal vertebral segmentation in spondylocostal dysostosis. Hum Mol Genet. 2011 Mar 1;20(5):905-16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5790062
Gene symbol: Dll1
Gene name: delta like canonical Notch ligand 1
Antibody: PGPM-1F9
Detection system: Secondary antibody
Results
Specimen 1H: embryonic day 9.5 (more )
Note: Freshly dissected unfixed non-permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 1H Dll1 Expression is in the presomitic mesoderm in this non-permabilized specimen.
Specimen 1I: embryonic day 9.5 (more )
Note: Freshly dissected unfixed permeabilized specimen. After primary antibody incubation specimens were fixed in 4% paraformaldehyde.
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 1I Dll1 Expression is in the presomitic mesoderm in this permabilized specimen.
Specimen 3H: embryonic day 9.5 (more )
Note: (I) is a higher magnification of (H), inset is a higher magnification of the boxed region. Double labeled: green - Dll1; red - Notch1 (assay MGI:5790067).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3I merge , 3I Dll1 , 3H merge , 3H Dll1 Expression is in the rostral presomitic mesoderm.
Specimen 3J: embryonic day 9.5 (more )
Note: Triple labeled: green - Dll1; red - Notch1 (assay MGI:5790067); blue - Dll3 (assay MGI:5790068).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 3J Expression is in the rostral presomitic mesoderm.
Specimen 4E: embryonic day 9.5 (more )
Note: (G and H) are higher magnifications of (E). Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4E merge , 4G Dll1 , 4G merge , 4H , 4E Dll1 Expression is in the rostral presomitic mesoderm and is strongest at its rostral limit.
TS15: somite Absent 4G Dll1 , 4H , 4E merge , 4G merge , 4E Dll1
Specimen 4I: embryonic day 9.5; Dll3tm1Rbe/Dll3tm1Rbe (more )
Note: (J) is a higher magnification of (I). Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 4J Dll1 , 4I Dll1 , 4I merge , 4J merge Expression is in the rostral presomitic mesoderm.
Specimen S3a: embryonic day 9.5 (more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3a merge , S3a Dll1 Expression is in the rostral presomitic mesoderm.
Specimen S3b: embryonic day 9.5 (more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted S3b merge , S3b Dll1 Expression is in the rostral presomitic mesoderm.
Specimen S3c: embryonic day 11.5 (more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3c merge , S3c Dll1 Expression is in the rostral presomitic mesoderm.
Specimen S3d: embryonic day 11.5 (more )
Note: Triple labeled: blue - Dll1; green - Dll3 (assay MGI:5790068); red - cleaved Notch1 (assay MGI:5790075).
Structure Level Pattern Image Note
TS19: tail unsegmented mesenchyme Present Regionally restricted S3d Dll1 , S3d merge Expression is in the rostral presomitic mesoderm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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