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Gene Expression Data
Assay Details
Assay
Reference: J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800404
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: Anti-Sycp3
Detection system: Secondary antibody
Results
Specimen S17A +/+ Leptotene: postnatal month 8 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Leptotene Merge , S17A_+/+ Leptotene Sycp3
Specimen S17A +/+ Zygotene: postnatal month 8 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Zygotene Sycp3 , S17A_+/+ Zygotene Merge
Specimen S17A +/+ Pachytene: postnatal month 8 (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_+/+ Pachytene Sycp3 , S17A_+/+ Pachytene Merge
Specimen S17A -/- Leptotene: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_-/- Leptotene Sycp3 , S17A_-/- Leptotene Merge
Specimen S17A -/- Zygo. Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_-/- Zygo. Like Sycp3 , S17A_-/- Zygo. Like Merge
Specimen S17A -/- Zygo Like: postnatal month 8; Top6blem1Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - Dmc1 (assay MGI:5800403). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S17A_-/- Zygo Like Sycp3 , S17A_-/- Zygo Like Merge
Specimen S18 +/+ Leptotene: postnatal day 30 (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18+/+ Leptotene Sycp3 , S18+/+ Leptotene Merge
Specimen S18 +/+ Zygotene: postnatal day 30 (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18+/+ Zygotene Merge , S18+/+ Zygotene Sycp3
Specimen S18 +/+ Pachytene: postnatal day 30 (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18+/+ Pachytene Sycp3 , S18+/+ Pachytene Merge
Specimen S18 -/- Leptotene: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18-/- Leptotene Merge , S18-/- Leptotene Sycp3
Specimen S18 -/- Zygo Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18-/- Zygo Like Merge , S18-/- Zygo Like Sycp3
Specimen S18 -/- Zygo. Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18-/- Zygo. Like Merge , S18-/- Zygo. Like Sycp3
Specimen S18 -/- Zygo. Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Sycp3; green - H1f6 (assay MGI:5800422). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present S18-/- Zygo. Like Sycp3 , S18-/- Zygo. Like Merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory