Gene Expression Data
Assay Details
Assay
|
Reference:
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J:231834
Vanhoutteghem A, et al., The importance of basonuclin 2 in adult mice and its relation to basonuclin 1. Mech Dev. 2016 May;140:53-73
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5805472
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Gene symbol:
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Bnc1
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Gene name:
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basonuclin zinc finger protein 1
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Results
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Specimen
2A:
postnatal newborn
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Specimen
2C/E:
postnatal newborn
(more )
Note: Double labeled: red - Bnc1; green - Sycp3 (assay MGI:5805476).
Specimen
2C/E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Bnc1; green - Sycp3 (assay MGI:5805476).
|
Specimen
2F:
postnatal day 60
(more )
Note: Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 60
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
|
Specimen
2G:
postnatal day 60
(more )
Note: Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
Specimen
2G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 60
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Bnc1; red - Vasa (Ddx4 assay MGI:5805477); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: germ cell of ovary |
Strong |
Regionally restricted |
2G
|
Abundant expression in nuclei of oocytes in follicles.
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Specimen
3F/H:
postnatal day 8
(more )
Note: Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
Specimen
3F/H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 8
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
3F
,
3H
|
Expression in a substantial number of tubular cells, mostly undifferentiated spermatogonia.
|
TS28: spermatogonium |
Present |
Regionally restricted |
3H
,
3F
|
Expression in undifferentiated and differentiating spermatogonia.
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Specimen
3I:
postnatal day 15
(more )
Specimen
3I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: germ cell of testis |
Present |
Regionally restricted |
3I
|
Expression confined to spermatogonia resting on the basal membrane.
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Specimen
3K/M:
postnatal day 30
(more )
Note: Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
Specimen
3K/M:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 30
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Bnc1; green PLZF (Zbtb16 assay MGI:5805478).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatogonium |
Present |
Regionally restricted |
3K
,
3M
|
Expression in undifferentiated and differentiating spermatogonia. No expression in meiotic or postmeiotic cells.
|
Specimen
5I:
postnatal newborn
(more )
Specimen
5I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: stomach wall |
Present |
Regionally restricted |
5I
|
Expression in outer surface of stomach.
|
Specimen
5K:
postnatal newborn
(more )
Specimen
5K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: small intestine |
Present |
Regionally restricted |
5K
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Expression localized in serosal coat of small intestine. No expression in smooth muscle layers.
|
Specimen
6B:
postnatal newborn
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6I/J:
postnatal newborn
(more )
Note: Triple labeled: red - Bnc1; green - Bnc2 (assay MGI:5805469); blue - DAPI.
Specimen
6I/J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Bnc1; green - Bnc2 (assay MGI:5805469); blue - DAPI.
|
Specimen
11B:
embryonic day 16.5
(more )
Specimen
11B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: palate epithelium |
Present |
Regionally restricted |
11B
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In an anterior section, expression specifically in medial part of oral epithelium.`
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Specimen
11C:
embryonic day 16.5
(more )
Specimen
11C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: palate epithelium |
Present |
Regionally restricted |
11C
|
In a more posterior section, expression in epithelial triangle at the midline, where palatal shelves have just fused.
|
Specimen
12H:
embryonic day 16.5
(more )
Specimen
12H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: palate epithelium |
Present |
Regionally restricted |
12H
|
Expression confined to epithelium on oral side.
|
Specimen
S1I:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
S1J:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Mutant Allele(s):
|
Bnc2Gt(pU21)18Imeg/Bnc2Gt(pU21)18Imeg
|
Age:
|
postnatal newborn
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|