Ank3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:214712 Le Bras B, et al., In vivo assembly of the axon initial segment in motor neurons. Brain Struct Funct. 2014 Jul;219(4):1433-50
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5828210
Gene symbol:	Ank3
Gene name:	ankyrin 3, epithelial

Antibody:	anti-AnkG
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

Specimen 5a1: embryonic day 10.5 (more

)
Note: Double labeled: green - Ank3; red - PanNav (all Nav alpha isoforms).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	5a3 , 5a1	Expression is in the motor neuron axons extending from the spinal cord.

Specimen 5b1: embryonic day 11.5 (more

)
Note: Double labeled: green - Ank3; red - PanNav (all Nav alpha isoforms).

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	5b3 , 5b1	Expression is in the proximal motor neuron axons.

Specimen 5c1: embryonic day 12.5 (more

)
Note: Double labeled: green - Ank3; red - PanNav (all Nav alpha isoforms).

Structure	Level	Pattern	Image	Note
TS20: basal columns	Present	Regionally restricted	5c3 , 5c1	Expression is in the proximal parts of the motor neuron axons.

Specimen 5d1: embryonic day 13.5 (more

)
Note: Double labeled: green - Ank3; red - PanNav (all Nav alpha isoforms).

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	5d3 , 5d1	Expression is in the proximal parts of the motor neuron axons.

Specimen 7a1: embryonic day 10.5 (more

)
Note: Double labeled: green - Ank3; red - Sptbn4 (assay MGI:5828270).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	7a3 , 7a1	Expression is in the motor neuron axons.

Specimen 7b1: embryonic day 11.5 (more

)
Note: Double labeled: green - Ank3; red - Sptbn4 (assay MGI:5828270).

Structure	Level	Pattern	Image	Note
TS19: neural tube lateral wall	Present	Regionally restricted	7b3 , 7b1	Expression is in the more proximal parts of the motor neuron axons.

Specimen 7c1: embryonic day 13.5 (more

)
Note: Double labeled: green - Ank3; red - Sptbn4 (assay MGI:5828270).

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	7c3 , 7c1	Expression is in the proximal axons of motor neurons.

Specimen 7d1: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	7d1	Expression is in the more proximal parts of the motor neuron axons.

Specimen 7d2: embryonic day 12.5; Sptbn4^{Gt(ROSABetageo)62Sor}/Sptbn4^{Gt(ROSABetageo)62Sor} (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	7d2	Expression in this mutant is the same as in wild-type.

Specimen 7e1: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	7e1	Expression is in the proximal axons of motor neurons.

Specimen 7e2: embryonic day 13.5; Sptbn4^{Gt(ROSABetageo)62Sor}/Sptbn4^{Gt(ROSABetageo)62Sor} (more

)

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	7e2	Expression in this mutant is the same as in wild-type.

Specimen 8a1: embryonic day 10.5 (more

)
Note: Double labeled: green - Ank3; red - Ank2 (assay MGI:5828282).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	8a1 , 8a3	Expression is in the motor neuron axons extending from the spinal cord.

Specimen 8b1: embryonic day 13.5 (more

)
Note: Double labeled: green - Ank3; red - Ank2 (assay MGI:5828282).

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	8b3 , 8b1	Expression is in the proximal parts of the motor neuron axons.

Specimen 8c1: embryonic day 10.5 (more

)
Note: Double labeled: green - Ank3; red - Sptan1 (assay MGI:5828289).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	8c1 , 8c3	Expression is in the motor neuron axons extending from the spinal cord.

Specimen 8d1: embryonic day 13.5 (more

)
Note: Double labeled: green - Ank3; red - Sptan1 (assay MGI:5828289).

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	8d1 , 8d3	Expression is in the proximal parts of the motor neuron axons.

Specimen 8e1: embryonic day 10.5 (more

)
Note: Double labeled: green - Ank3; red - Sptbn2 (assay MGI:5828291).

Structure	Level	Pattern	Image	Note
TS17: neural tube lateral wall	Present	Regionally restricted	8e3 , 8e1	Expression is in the motor neuron axons extending from the spinal cord.

Specimen 8f1: embryonic day 13.5 (more

)
Note: Double labeled: green - Ank3; red - Sptbn2 (assay MGI:5828291).

Structure	Level	Pattern	Image	Note
TS21: ventral grey horn	Present	Regionally restricted	8f1 , 8f3	Expression is in the proximal parts of the motor neuron axons.

Specimen S1a: embryonic day 16.5 (more

)

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Present		S1a1

Specimen S1d: embryonic day 16.5 (more

)
Note: Triple labeled: blue - Ank3 (rabbit antiserum); red - Ank3 (guinea pig antibody; assay MGI:5828213); green - Ank3 (mouse antibody; assay MGI:5828294).

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Present		S1d4 , S1d1

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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