Gene Expression Data
Assay Details
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Assay
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Reference:
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J:173765
Mustata RC, et al., Lgr4 is required for Paneth cell differentiation and maintenance of intestinal stem cells ex vivo. EMBO Rep. 2011 Jun;12(6):558-64
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5883548
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Gene symbol:
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Lgr4
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Gene name:
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leucine-rich repeat-containing G protein-coupled receptor 4
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1A E15:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: ileum |
Present |
Regionally restricted |
1A E15 X-gal
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Expression in the pseudo-stratified epithelium.
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| TS23: small intestine smooth muscle |
Present |
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1A E15 X-gal
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Authors also report expression in mesenchymal or smooth muscle cells.
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Specimen
1A P0:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: ileum |
Present |
Regionally restricted |
1A P0 X-gal
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Expression in intervillus progenitors.
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| TS27: small intestine smooth muscle |
Present |
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1A P0 X-gal
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Authors also report expression in mesenchymal or smooth muscle cells.
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Specimen
1A Adult:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ileum |
Present |
Regionally restricted |
1A Adult X-gal
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Expression all along the crypts, above the Paneth cell zone, in the transit-amplifying cell region, in crypt basal columnar cells, and between Paneth cells.
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| TS28: intestinal villus |
Absent |
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1A Adult X-gal
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| TS28: small intestine smooth muscle |
Strong |
Regionally restricted |
1A Adult X-gal
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Strong expression in smooth muscle layers.
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Specimen
1A Adult +fast red:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Specimen
1C:
postnatal adult; Lgr4Gt(LST020)Byg/Lgr4 +
(more  )
Note: Triple labeled: green - beta-gal (Lgr4 reporter); red - Lyz1 (assay MGI:5883556); blue - DAPI.
Specimen
1C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Note:
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Triple labeled: green - beta-gal (Lgr4 reporter); red - Lyz1 (assay MGI:5883556); blue - DAPI.
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Specimen
S1A P0 Duodenum:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: duodenum |
Present |
Regionally restricted |
S1A P0 Duodenum
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Authors report expression pattern similar to that seen for the ileum.
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Specimen
S1A Adult Duodenum:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: duodenum |
Present |
Regionally restricted |
S1A Adult Duodenum
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Authors report expression pattern similar to that seen for the ileum.
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Specimen
S1A Adult Colon:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: colon |
Present |
Regionally restricted |
S1A Adult Colon
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Authors report expression pattern similar to that seen for the ileum.
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Specimen
S1B:
postnatal day 15; Lgr4Gt(LST020)Byg/Lgr4 +
(more )
Note: Triple labeled: green - beta-gal (Lgr4 reporter); red - Vim (assay MGI:5883558); blue - DAPI.
Specimen
S1B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lgr4Gt(LST020)Byg/Lgr4+
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Note:
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Triple labeled: green - beta-gal (Lgr4 reporter); red - Vim (assay MGI:5883558); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: ileum |
Present |
Regionally restricted |
S1B beta-gal/DAPI
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S1B merge
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Expression in intestinal subepitheliual myofibroblasts and enteric neurons.
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Analysis Tools